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Why important to use Agar Deep Stab medium soon after preparation?
Wiki User
∙ 11y ago
Over time, free oxygen will diffuse into the agar.
Wiki User
∙ 11y ago
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What is the advantage of an agar slant versus an agar deep?
agar is botained from a red alga called gelidium
What is agar deep?
Agar deep refers to a measure used with microbes. It is a measurement of how much gas a microbe requires for survival.
What is slant broth and deep?
a slant broth is a type of inoculating agar to gather bacterial data from.
Use of agar slant?
An agar slant is when a test tube is filled with liquid agar and allowed to cool and harden at an angle (slant). Agar is mixed with other nutrients to provide a medium for which bacteria can grow on.
Are bacteria growing in the semi solid agar deep motile?
Yes. If the bacteria has spread from the inoculation point, the bacteria is motile. It will look like a upside down Christmas tree.
When inoculating agar deep stabs why do you have to insert and remove the needle along the same stab line?
To minimize the introduction of unwanted oxygen into the medium.
What is the advantage of an agar slant versus an agar deep?
agar is botained from a red alga called gelidium
What is agar deep?
Agar deep refers to a measure used with microbes. It is a measurement of how much gas a microbe requires for survival.
Inoculating an agar deep tube?
inoculation needle
Why must a straight inoculating needle be used when inoculating an agar deep tube?
This is important to prevent the inoculating needle from becoming stuck in the agar, taking out pieces of agar while trying to remove the instrument. This agar will get into the inoculum when sterilizing the needle on the flame, causing contamination to your sample.
What does John Dillinger's voice sound like?
Medium deep not to deep but it was somewhat deep
What is slant broth and deep?
a slant broth is a type of inoculating agar to gather bacterial data from.
How do you differentiate between E. coli and salmonella?
E.coliIn stool samples microscopy will show Gram negative rods, with no particular cell arrangement. Then, either MacConkey agar or EMB agar (or both) are inoculated with the stool. On MacConkey agar, deep red colonies are produced as the organism is lactose positive, and fermentation of this sugar will cause the medium's pH to drop, leading to darkening of the medium. Growth on Levine EMB agar produces black colonies with greenish-black metallic sheen. This is diagnosic of E. coli.SalmonellaCulture is microbial growth on or in a nutritional solid or liquid medium; increased numbers of organisms simplify identification. Culture also facilitates testing of antimicrobial susceptibility.Communication with the laboratory is essential. Although most specimens are placed on general purpose media (eg, blood or chocolate agar), some pathogens require inclusion of specific nutrients and inhibitors or other special conditions
Why did you stab the peptone iron agar instead of inoculating only the surface?
Because the peptone iron agar is used to detect ANAEROBIC bacteria. If you stab it deep into the agar you allow the bacteria to grow in the absence of oxygen. If you only inoculated the surface the bacteria wouldn't grow.
What type of pocket should a midfield lacrosse player have?
Medium to deep
Why does a straight inoculating needle used to inoculate an agar deep tube?
because you want to introduces as little air as possible
What are the uses of deep agar?
Agar deeps are used to see whether an organism requires oxygen to grow. If there is spreading growth only at the bottom of the tube, the organism is an obligate anaerobe (meaning it cannot tolerate oxygen). If there is growth only at the surface of the agar, the organism is an obligate aerobe (it cannot grow without oxygen). And if there is growth all along the point of innoculation, the organism is a facultative anaerobe and can survive either way.
