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To separate and analyze DNA fragments and protein fragments by weight. If you have digested some bacterial DNA, for instance, then you can tell by running the fragmented DNA in the gel whether you have digested the correct base length.

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Paul Wyman

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1y ago
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9y ago

Gel electrophoresis is an important part of identifying DNA molecules. It helps scientists see the difference between organisms such as animals, humans, plants, and other living organisms.

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Q: Why is gel electrophoresis important?
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What are the pros and cons of gel electrophoresis?

Pros: The detection of DNA, RNA and proteins can be done using gel electrophoresis. Gel electrophoresis does not require a large amount of starting material. Cons: difficult to extract samples for further analysis. Harmful materials.


Are restriction enzymes used in gel electrophoresis?

For DNA gel electrophoresis, yes. Once the DNA is cut up into different-sized fragments, they can be electrophoresed to separate bands.


How does gel electrophoresis is used to make a DNA fingerprint?

Gel electrophoresis separates DNA fragment on the basis of their size. In DNA fingerprinting or DNA typing given sample is cut up with restriction enzymes and run through electrophoresis and results are analyzed to check for DNA polymorphism between the given sample and a sample form suspect. In nutshell gel electrophoresis is boon for the people in forensics.


What is the conclusion on the application of gel electrophoresis?

One of the Conclusion of electrophoresis is Visualization of the DNA size. Second is Sequencing the length of DNA of the body.


What is th blue dye added to the sample before the electrophoresis is performed?

In biochemistry labs, the traditional answer for a protein gel (polacrylamide gel electrophoresis) is bromphenol blue. For a DNA gel (agarose gel electrophoresis), traditionally the same dark blue dye bromphenol blue was combined with the lighter, slower migrating blue dye xylene cyanol. Oftentimes nowwe only use the bromphenol blue, or even substitute for it with Orange G, which is a UV-transparent dye that more easily enables the visualization of smaller molecular weight nucleic acids that migrate in the same region.

Related questions

What is used to sort DNA into different lenghts?

Gel Electrophoresis


what is the gel in Gel Electrophoresis?

For larger molecules like proteins we use polyacrylamide gel electrophoresis (PAGE). For smaller pieces like DNA we use agarose gel electrophoresis


Can a gel electrophoresis be performed both horizonnally and vertically?

yes for example 2D gel electrophoresis


A technique that can be used to compare the DNA of two or more plants is?

Gel electrophoresis


What is a common name for gel electrophoresis?

gel


Why was it important to stop the gel electrophoresis before the dye ran off the edge of the gel?

You would lose your DNA.


Where can one learn more on gel electrophoresis?

To learn more about gel electrophoresis, one can Google it. There is also a whole Wikipedia article dedicated to gel electrophoresis, and it happens to be quite informative.


What has the author A J Houtsmuller written?

A. J. Houtsmuller has written: 'Agarose-gel-electrophoresis of lipoproteins' -- subject(s): Blood protein electrophoresis, Electrophoresis, Gel electrophoresis, Lipoproteins


Process that restriction fragments of DNA are separated from each other by the use of electricity?

The process is referred to as gel electrophoresis. This is an analytical process where DNA fragments can be separated based on size within a gel under the influence of an electric field


Can gel electrophoresis be used to determine the purity of an enzyme?

Yes it can be done if you use Poly-Acrylamide Gel Electrophoresis.


Difference between vertical and horizontal gel electrophoresis?

Horizantal gel electrophoresis is generally used for RNA/DNA based studies, while vertical gel electrophoresis is used for protein based studies.


What is the function of the gel electrophoresis?

Gel electrophoresis is an analytical method used to separate DNA, RNA or proteins based on size