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it is used to dye materials like silk,leather and paper.
And also to:
As mineral specimens, an important ore of copper, as an ornamental stone, a pigment and for jewelry.
- Natural malachite mineral Specimens
Earlene Ritchie
James William
No, malachite green is not used in the Gram stain. The Gram stain is a commonly used laboratory technique in microbiology to differentiate bacteria into two major groups: Gram-positive and Gram-negative. The stain involves a series of steps, including applying crystal violet (a purple dye), iodine, ethanol or acetone, and a counterstain such as safranin.
Malachite green, on the other hand, is a green synthetic dye that is not typically used in the Gram stain. It is commonly used in other staining methods, such as the spore stain, which is used to detect the presence of bacterial endospores.
It's important to note that while malachite green has been used as a general biological stain, its usage and availability may vary depending on the specific protocols and techniques employed in different laboratories. Always refer to the specific staining procedure or consult with a microbiologist or laboratory specialist for accurate and up-to-date information regarding staining techniques.
Wiki User
∙ 12y agoit is used to dye materials like silk,leather and paper.
And also to:
As mineral specimens, an important ore of copper, as an ornamental stone, a pigment and for jewelry.
- Natural malachite mineral Specimens
Wiki User
∙ 9y agoMalachite green is used as a dye because it works well with dying certain materials, cells, and minerals. It can easily dye paper, silk, leather, ores, endospores, and more.
Wiki User
∙ 11y agoWiki User
∙ 15y agoMalachite is a nonmetallic mineral that is used for ore of copper for pipes, electrical circuits, coins, ammunition, and gemstones.
Wiki User
∙ 13y agoIt is a spore former and a local anticeptic
Wiki User
∙ 11y agoyes
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Explain the function of water in spore staining?
Water removes (decolorizing agent) the Malachite green from all parts of the bacteria excluding the spore. The spore appears green when examined.
What dye is used to stain endospores?
To differentiate the spores from other similar looking structures in the cell that don't stain as the spores do.
Assume that during the performance of this exercise you made several errors in your spore staining procedure?
Assume that during the performance of this exercise you made several errors in your spore-staining procedure. In each of the following cases indicate how your microscopic observations would differ from those observed when the slides were prepared correctly . A. you used acid-alcohol as the decolorizing agent . B. you used safranin as the primary stain and malachite green as the counterstain C. you did not apply heat during the application of the primary stain · A. Normally tap water is used as the decolorizing agent to wash off excess stain. When you use acid-alcohol, it decolorizes the cells and the stain is removed. · B.When you use safranin as the primary stain and malachite green as the secondary stain, the cells will stain green and the spores will stain red. · C.When heat is not applied during the application of the primary stain, the spores are not stained and they appear colorless.
Is malachite green basic dye?
yes malachite is a basic dye and used in dyeing of acrylic fabric. it is also known as basic green.
Why is the counter stain for gram stain procedure differ from the counter stain used for acid fast procedure?
Safranin
Why is it necessary to heat the malachite green?
Malachite green is used as a primary stain in bacteria. It is necessary to heat the malachite green in order to penetrate the endospore of the bacteria.
What dye is used to penetrate and stain an endospore?
Malachite green
What is the stain used to staines the cell membrane?
we can use feulgen's method ,in which malachite green stain is used to stain the nucleus
What are the properties that differentiate the spore from the vegetative cell?
In the spore stain malachite green is used as a primary stain. This is driven into the cell by heat because of the impermeability of the spore. The stain is allowed to sit for 30 mins to make sure it gets in to the endospores.The stain is then washed and counterstained with safranin red. The endospores retain the green colour from malachite green and of course appear green under the microscope. Whereas the vegetative cells will appear red.
Explain the function of water in spore staining?
Water removes (decolorizing agent) the Malachite green from all parts of the bacteria excluding the spore. The spore appears green when examined.
What dye is used to stain endospores?
To differentiate the spores from other similar looking structures in the cell that don't stain as the spores do.
What is the gram stain of H5N1?
The Gram stain is used for bacteria and not for viruses.
Assume that during the performance of this exercise you made several errors in your spore staining procedure?
Assume that during the performance of this exercise you made several errors in your spore-staining procedure. In each of the following cases indicate how your microscopic observations would differ from those observed when the slides were prepared correctly . A. you used acid-alcohol as the decolorizing agent . B. you used safranin as the primary stain and malachite green as the counterstain C. you did not apply heat during the application of the primary stain · A. Normally tap water is used as the decolorizing agent to wash off excess stain. When you use acid-alcohol, it decolorizes the cells and the stain is removed. · B.When you use safranin as the primary stain and malachite green as the secondary stain, the cells will stain green and the spores will stain red. · C.When heat is not applied during the application of the primary stain, the spores are not stained and they appear colorless.
Is malachite green basic dye?
yes malachite is a basic dye and used in dyeing of acrylic fabric. it is also known as basic green.
What are some practical uses for malachite?
Malachite is a green mineral which has many uses. It has been used as a gemstone in jewelery, it is used in dyes and it has also been used in witchcraft.
In spore staining what would happen it you used safarin as the primary stain and malachite green as the counterstain?
The spore would appear to be red as the safranin is heat driven into the many layers of the spore, however, as Malachite green has a weak affinity and is water soluble, it will not likely bind to the spore wall or the cell wall. You might have traces of green on the slide if any, but it will be very little. Your vegetative cells will be pink as well.
What do you make from malachite?
Malachite makes a green dye, which is used to colour many green chemicals, such as cleaners. The dye is extremely potent - a tiny pinch of the powder can colour a large vat of chemical.
