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limiting dilution is a method used to have a single cell / well in a tissue culture plate. and why we need a single cell / well ...to ensure that we have single clone of antibody producing B cell as during any immuneresponse a mixed of different B clone will produce to make antibodies against foreign antigen even during the 2ndary immune response the antibodies produced will have different structure in the variable region because of somatic hypermutation process during affinity maturation at the germinal center. Thus, we do a limiting dilution to study each B cell clone antibody produced individually and test its specificity by ELISA, which can be easy to detect the best affinity binder clone and chose it for further steps.
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Different types of fusion technique used in hybridoma technique?
The different types of fusion technique used in Hybridoma formatio are: 1. fusion mediated by PEG. 2. fusion mediated by DMSO. 3. Electrofusion 4. Viral Transformation (viral mediated fusion) using EB Virus, Sandai Virus.
Why is the term limiting used to describe the limiting reactant?
The term "limiting" is used because the limiting reactant limits the amount of product produced.
Is dilution an acceptable way to dispose of the used copper chloride solution?
Is dilution an acceptable way to dispose of the used copper chloride solution
What is the formula used to calculate dilution ratios?
A dilution ratio is normally used for a mixture of two fluids: an active component and a carrier solvent. The dilution ratio is the ratio of the volume of the solvent to the volume of the active component.
Is dilution an acceptable way to dispose of the used copper chloride solution Explain?
Is dilution an acceptable way to dispose of the used copper chloride solution
What best describes a stock solution that is used in a dilution?
A stock solution that is used in a dilution is known as a dilute solution. A dilute solution reduces the Ph of a chemical.
Which solutions are used most often for ivpb dilution?
vancomycin
What is the reactant called that gets used up first?
The Limiting Reactant is the reactant that runs out first in a reaction.
How does nitrogen dilute oxygen?
nitrogen is used in dilution of activity of which gas
In the calculation trypan blue exclusion assayof haemocytometer why dilution factor 2 is used not 0.5?
Because you probably used a 2-fold dilution. So you need to multiply your count by 2 to get the ACTUAL amount.
What is the thing called that is completely used up in a reaction?
The limiting reagent (aka limiting reactant) is the substance which is completely used in a chemical reaction.
Why does the amount of product formed is directly proportional to the amount of limiting reactant used?
because the limiting reactant is not in excess so it's all used up . as the limiting reactant is used up the reaction stops so no more product can be made.
