You should have already used the Coarse adjustment on 10x so when you switch to 40x, all you need to do is fine adjust it. Plus, you don't want to break it by turning the Coarse up all the way.
you can not used the course objective knob for 40x objective because it can break the slides, and possibly damage the lens.
The coarse and fine adjustment knobs can be used with the low power objective.
The fine adjustment knob is used with the power objective on the microscope. Since the objective is already at the highest objective possible for that microscope, using the coarse adjustment knob would focus to much on the object making the details harder to read. Using the fine adjustment knob, you are able to see the fine details within the object or organism you are studying. The higher the power, the finer the knob and the lower the objective, the coarser the knob and objective.
the stage and the objective lens
coarse focus. This was by Joseph, hi R.C.S
Ideally, the microscope should be returned to its lowest-magnification objective when the user finishes his or her work. Because not all users abide by this unwritten law, it's best-practice to use the "Fine Focus" knob (the smaller diameter of the two, usually farthest from the body of the scope) when first sitting at a microscope you don't own. This will prevent the sample from breaking because you've accidentally moved the stage (the moveable, horizontal part of the microscope on which your specimen sits) too rapidly after the last use took off, leaving the objective at 40x (400x total magnification in most instances). If you verify that the objective turret is set such that the lowest magnification objective is in place, you can usually safely use the larger knob located closer to the body of the 'scope (but on the same axis as the fine knob) to bring your sample into focus. As you move to higher powers, the fine is all the adjustment you should need. COARSE, you didn't even end up answering the question previous writter! seriously!!!!! i agree with you 2nd writer!!!!
The coarse and fine adjustment knobs can be used with the low power objective.
The fine adjustment knob is used with the power objective on the microscope. Since the objective is already at the highest objective possible for that microscope, using the coarse adjustment knob would focus to much on the object making the details harder to read. Using the fine adjustment knob, you are able to see the fine details within the object or organism you are studying. The higher the power, the finer the knob and the lower the objective, the coarser the knob and objective.
the stage and the objective lens
coarse focus. This was by Joseph, hi R.C.S
First focus the microscope on the lowest objective using first the coarse djustment knob and then the fine adjustment knob. Once you have it in focus, move on and do the same for the next highest objective. Continue this process until you reach the highest objective and focus it in the same fashion.
Ideally, the microscope should be returned to its lowest-magnification objective when the user finishes his or her work. Because not all users abide by this unwritten law, it's best-practice to use the "Fine Focus" knob (the smaller diameter of the two, usually farthest from the body of the scope) when first sitting at a microscope you don't own. This will prevent the sample from breaking because you've accidentally moved the stage (the moveable, horizontal part of the microscope on which your specimen sits) too rapidly after the last use took off, leaving the objective at 40x (400x total magnification in most instances). If you verify that the objective turret is set such that the lowest magnification objective is in place, you can usually safely use the larger knob located closer to the body of the 'scope (but on the same axis as the fine knob) to bring your sample into focus. As you move to higher powers, the fine is all the adjustment you should need. COARSE, you didn't even end up answering the question previous writter! seriously!!!!! i agree with you 2nd writer!!!!
Coarse focus on a microscope is used only when you are using the lowest objective lens. You use it to get the object magnified mostly. The fine focus is then used to adjust the resolution to the viewer's eyesight.
When the high-powered objective is in position.
The fine focus knob is used to bring an object into fine focus, while the course focus knob is used to bring an object into approximate focus. The course focus knob should only be used on the low-power and scanning objectives of the light microscope. Using it on the high power microscope is okay, but you should be very careful. It is no recommended to use the course adjustment knob on the oil-emersion objective as it is so close to the stage and could lead to a potential break in the slide. Fine focus adjustment knob is useful to bring out the fine details in the specimen on your slide. Can be used on any of the objectives, but most receptive on the high-power and oil-emersion objectives.
Simply because the fine focus moves too quick to get the object focused. Chances are you will miss the focus point.
The electron microscope has an eyepiece lens, tube, revolving turret, coarse focus, fine focus, objective lens, rack stop, arm, stage, and an illuminator.
Coarse focus on a microscope is used only when you are using the lowest objective lens. You use it to get the object magnified mostly. The fine focus is then used to adjust the resolution to the viewer's eyesight.