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Because solutions with "free" amino acids don't have peptide bonds and peptide bonds need to be present in order for the test to be positive.

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What type of food will give a negative Iodine test and a positive Biuret Solution test?

An iodine test is used to test for the presence of starch (or polysaccharides, specifically amylose or amylopectin). A Biuret solution test is used as an indicator for peptide bonds within proteins. Therefore, if you get a negative iodine test and a positive Biuret test, you would probably be testing a animal food source (beef, chicken, pork). Overall, any food that doesn't have starch present within it would be appropriate to use. Hope this helps!


What is the reagent used to test proteins?

The reagent commonly used to test for proteins is Biuret reagent. It reacts with peptide bonds in proteins to form a color change, ranging from blue (negative) to purple (positive), indicating the presence of proteins in the sample.


What is the name of the test for proteins?

The test for proteins is called the Biuret test. This test is based on the principle that proteins react with copper sulfate in an alkaline solution to produce a violet color.


What might Biuret reagent test in a practical real life situation?

Biuret reagent is commonly used in laboratories to test for the presence of proteins. In practical real life situations, the Biuret test can be used to identify the protein content in food products, such as milk, eggs, and meat. It can also be used in medical diagnostics to detect protein abnormalities in bodily fluids like urine or blood.


What color is a positive test for protein?

A positive test for protein typically appears as a violet or purple color when using chemical indicators such as Biuret reagent. This color change indicates the presence of proteins in the test sample.

Related Questions

Does phenol give a positive biuret test?

No, phenol does not give a positive biuret test. The biuret test is used to detect the presence of proteins, not phenol. Phenol is a type of organic compound that does not contain the peptide bonds that the biuret test reacts with.


Which sample gives a negative Biuret test and why?

Gelatin and egg albumin.


What is biuret test look like negative?

In a negative Biuret test, the solution will remain its original color (usually blue) and there will be no color change to pink or purple, indicating the absence of proteins in the sample.


Does pepsin test positive in biuret test?

Pepsin does not test positive in the biuret test. The biuret test is used to detect the presence of proteins in a solution, which contain peptide bonds. Pepsin is an enzyme that breaks down proteins into smaller peptides, so it does not give a positive result in the biuret test.


Will glutamic acid give a positive biuret test?

Amino acids doesn't react with the biuret reagent.


How many of these chemical groupings must be present in a molecule to give a positive biuret test?

At least two peptide bonds must be present in a molecule to give a positive biuret test. The biuret reagent interacts with peptide bonds in proteins, forming a colored complex that can be detected visually.


What type of food will give a negative Iodine test and a positive Biuret Solution test?

An iodine test is used to test for the presence of starch (or polysaccharides, specifically amylose or amylopectin). A Biuret solution test is used as an indicator for peptide bonds within proteins. Therefore, if you get a negative iodine test and a positive Biuret test, you would probably be testing a animal food source (beef, chicken, pork). Overall, any food that doesn't have starch present within it would be appropriate to use. Hope this helps!


How many chemical grouping must be present in a molecule to give a positive Biuret Test?

You must have at least two, those that will form the peptide linkage (NH2 and COOH) since Biuret test is for detecting peptide linkages.


Would albumin give a positive biuret test?

Hey. okay.. the biuret test is a test for peptide bonds. when CuSO4 is added to KOH, an alkaline solution, nitrogen atoms from the peptide bond forms a purple compound with the Cu2+ ions. hope this helps! read it in a text book.


Would glucose give a positive result with the Biuret test?

No, glucose would not give a positive result with the Biuret test. The Biuret test is specific for detecting proteins, not sugars like glucose. It works by reacting with peptide bonds in proteins to form a colored complex.


What is the reagent used to test proteins?

The reagent commonly used to test for proteins is Biuret reagent. It reacts with peptide bonds in proteins to form a color change, ranging from blue (negative) to purple (positive), indicating the presence of proteins in the sample.


Is biuret test suitable to test the presence of proteins in the urine?

Yes, the biuret test will indicate the presence of peptides. If it turns violet, it is a positive result.