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How can you determine whether the zone of inhibition is due to death or the effect of the antibiotics?

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16y ago

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What is a inhibition?

A density dependent inhibition is when cells become more and more numerous, the required growth factors and nutrients become insufficient making cell growth difficult.


What if a agar plate is isolated and antibiotic added and result is no growth or inhibition?

This is basically ANTIBIOTIC SENSITIVITY TEST, to test whether the given organism is RESISTANT(no zone of inhibition) or SENSITIVE( zone of inhibition) to the given antibiotic.Zone of Inhibition Testing is a fast, qualitative means to measure the ability of an antimicrobial agent to inhibit the growth of microorganisms.The effectiveness is based upon the size of zone of inhibition,diffusability of antibiotic,size of inoculum,type of media used.example: bacillus organism is inoculated with both PENICILLIN and AMPICILLIN ,zone of inhibition is absent in case of penicillin and present in case of ampicillin, this shows that ampicillin (sensitive) worked effectively when compared to penicillin.


Define oligodynamic action?

its the microbial growth inhibition by heavy metal ions


What is it called when cell growth and division stops because the cells have run out of room?

Cell growth and division stops when cells become crowded or reach a certain density, which is known as contact inhibition. This mechanism helps control tissue growth and prevent overcrowding of cells.


How does bacterium help soils?

Some bacterium have the capability of nitrogen fixation. This is where nitrogen in the atmosphere, which is inert, is taken from a bacterium and fixed into a complex molecule. This is absorbed by plants and is used and growth and herbivores depend on plants for growth and carnivores depend on herbivores for growth. Most life depends on what microorganism produce as bi-products.


What is the recommended method for measuring the zone of inhibition in antimicrobial susceptibility testing?

The recommended method for measuring the zone of inhibition in antimicrobial susceptibility testing is to use a ruler to measure the diameter of the clear area around the antibiotic disk where bacterial growth is inhibited. This measurement helps determine the effectiveness of the antibiotic against the bacteria being tested.


What is the plural possessive for bacterium?

The plural form of the noun bacterium is bacteria.The plural possessive form is bacteria's.example: This will arrest the bacteria's growth.


Why is it important to first determine whether growth occurred in a differential medium such as starch agar before examining the plate for starch hydrolysis?

I do not know precisely what this is used for but starch agar is usually used to test whether the cultured cells produce amylase. In a teaching-experiment setting if no growth occurred then there really is no point to check whether the cells produced amylase or not since they probably didn't survive, and dead cells don't produce amylase. Any hydrolysis detected with iodine solution would be caused by stray enzymes such as those in a drop of saliva.i dont know go find out


What best describes contact inhibition?

Contact inhibition is a phenomenon where cells stop dividing when they come into contact with other cells. This process helps regulate cell growth and prevents overcrowding of cells, which can lead to uncontrolled growth and potential tumor formation. Contact inhibition plays a crucial role in maintaining tissue structure and homeostasis in multicellular organisms.


What is the meaning of angiogenesis?

The inhibition of formation of blood vessels feeding the tumor and contributing to tumor growth.


What is a bacterium that needs oxygen?

A bacterium that needs oxygen is called an aerobic bacterium


Why do you look at the turbidity when measuring the MIC?

Turbidity is used as an indirect measure of microbial growth in a culture. In the context of measuring Minimum Inhibitory Concentration (MIC), turbidity helps indicate the extent to which a substance inhibits microbial growth. Higher turbidity suggests more growth, while lower turbidity indicates inhibition, helping determine the MIC.