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What are the differences between streak plate technique and pour plate technique?
Wiki User
∙ 12y ago
Streak Plate:
Pure colonies of bacterial or other microorganisms can be obtained on petri dishes by streak plate. The microbial mixture is transfered to the edge of an agar plate with an inoculating loop or swab and then streaked out over the surface in one of several patterns. After the first sector is streaked in dish, the inoculating loop is sterlized and an inoculum for the second sector is obtained from the first sector. The same is done for third and fourth sector. Thus this is a dilution process. Eventually, very few cells will be on inoculating loop, a single cells will drop from it as it is rubbed along the agar surface. These develop into seprate colonies.
Pour Plate:
Extensively used with procaryotes and fungi, a pour plate also can yield isolated colonies. The original sample is diluted several times to reduce the microbial population sufficiently to obtain separate colonies when plating. Then small volumes of several diluted samples are mixed with liquid agar that has been cooled to about 45oC and the mixture are poured immediately into sterile culture dishes. Most bacteria and fungi are not killed by a brief exposure to the warm agar. After the agar has hardened each cell is fixed in place and forms an individual colony.
Wiki User
∙ 12y ago
Wiki User
∙ 14y agoIn pour plate technique the microoganisms are suspended in between the culture media because the inoculation is done when the media is cool enough not to denature microoganisms nor too hot to kill them,diluting them to distribute them then incubating them.Growth is observed suspended. On the other side the spread plate technique the microorganisms are on the surface and not suspended since we use the glass rod to inoculate.
Wiki User
∙ 9y agoSome advantages of pour plate technique are: it counts only living cells and it is a standardized test, which is used worldwide. Some disadvantages of this method are: it generally takes a long time to incubate bacteria and osmotic shocks can occur.
Wiki User
∙ 12y agoIn the pour plate, the microorganisms will grow within the gel that has been set, and in the spread-plate technique, growth will be on top of the agar gel where it has been spread.
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What is the differences between a streak plate technique and a serial dilution technique?
what is serial dilution and spread plate technique
1 how are microorganisms diluted and spread out to form individual colonies In the streak plate technique?
Bacterial mixture is transferred to the edge of an agar plate with an inoculating loop and then streaked out in one of several patterns. At some point, individual cells will be removed from the loop and will give rise to separate colonies.source: http://quizlet.com/17578430/micro-lab-unit-1ex-15-16-streak-plate-technique-flash-cards/
What is a streak plate?
A streak plate is a surface of unglazed ceramic, used to find the true color of a mineral specimen by drawing the specimen across it. The color of the resultant powder is referred to as the streak or streak color of a mineral.
What is the purpose of ethanol in the spread plate technique?
There are mainly two techniques in microbiology in bacterial cell inoculation. The first is when the colony is added to the plate and spread with a spreader across the entire plate in aseptic conditions. The second is called 16-streak and is used to isolate a single colony
If an inoculated plate had colonies between the streak lines what would you conclude?
It depends, if there is no growth or colony appearance on streak line and only it shows growth in b/w the streak line then it is certainly a contamination and if there are colonies on streak line and not ressemble with the streak culture then also it is a contamination but there can be a chance that colony appears due to some fault in streaking procedure and the inoculum drops between the streak line so it depends.
What is the differences between a streak plate technique and a serial dilution technique?
what is serial dilution and spread plate technique
What is the simplest technique for isolating bacteria in growth media is referred to as the?
Streak-plate method
What is the purpose of the streak for isolation?
By using streak plate technique to spread a clinical sample out on the surface of a growth medium individual types of bacteria can be isolated
What is the hardness of a porcelain streak plate?
The hardness of a porcelain streak plate will vary between 6.5-7.5 on the Mohs hardness scale.
Could some bacteria grow on the streak plate and not be seen using the pour plate technique?
Put simply - yes. Some strictly aerobic organisms will not grow in a pour plate. They may, however proliferate on a streak plate. Also consider the posibility of experimental error. The culture may have been added to the molten agar when it was too hot for the organisms to survive.
What is the purpose of the plate?
A streak plate is used to determine the streak colour of a mineral. You take the mineral and scratch it against the streak plate, and it will create a coloured streak on the plate. The streak is not always the same colour as the mineral itself.
Will diamond leave a streak on a streak plate?
Diamond will not leave a streak on a porcelain streak plate because diamond is harder than the streak plate. It will leave a scratch on the streak plate for the same reason.
What happens if you rub the mineral across a streak plate but the mineral does not leave a streak?
The lack of a streak would indicate that the mineral is harder than the streak plate, or the color of the streak is the same as the color of the streak plate.
How do you test out a streak?
by strecting it on a streak plate
Which is a good way to tell the difference between hematite and galena?
galena leave dark grey streak on streak plate where as hematite leave cherish red streak.
Can you show me a picture of a streak plate that has a streak from aquamarine on it?
I do not have a picture, but when you scratch aquamarine on a streak plate, the powder will be white colored.
Why is the streak-stab technique preferred rather than incubating the plates anaerobically?
The streak-stab technique is preferred over incubating the plates anaerobically because when isolating colonies allows biochemical testing to be performed. When the plate is incubated anaerobically it lacks oxygen and can not be biochemically tested.
