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Fermenting citrate

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Q: Why the slant portion is blue but the butt remains green at the microorganism grows on Simmon's citrate agar?
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Role of Citrate in glycolysis?

Citrate is a product / intermediate of the TCA Cycle (Which is responsible for breaking down Acetyl Coenzyme A ( Acetyl CoA ) into its final energy sources (1xGTP, 3xNADH, 1xFADH2) and byproducts CO2. Citrate generally remains in the Mitochondria (Where the TCA Cycle takes place), but may be transported to the cytosol. Citrate in the cytosol (The location of Glycolysis) acts to inhibit phosphofructokinase, which is an enzyme in the rate limiting step of Gl Save ycolysis involving the reaction of Fructose-6-phosphate to Fructose-1,6-bisphosphate.To summarize / Homework Answer: Increased cytosol levels of Citrate will decrease the rate of Glycolysis.

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