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It can be done all in one synthesis. However, there are 3 OHs on glycerol and thus 3 fatty acids required in total to build the triglyceride. There are thus 3 reactions in total that take place but all in one synthesis. This is similar to 3 molecules of NaOH reacting with one molecule of phosphoric acid.

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Q: How many dehydration synthesis reactions are required to attach fatty acid tails to a glycerol and build a triglyceride?
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