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This serves two functions; first, it stops the enzyme-catalyzed reaction by changing the pH to one unsuitable for the enzyme (the enzyme is denatured at high pH and so unable to function). Second, it deprotonates the p-nitrophenol to give the yellow colored p-nitrophenylate.

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12y ago
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Q: Why NaOH is used as stop solution to determine specific activity of alkaline phosphatase?
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