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Complementary base pairing occurs only between the probe and the target gene.

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14y ago

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Explain why a probe will hybridize to a target gene but not to any unrelated genes?

We design probes to be specific so that they will hybridize only to the target gene but not to random, unrelated genes. A probe hybridizes to a sequences to which it is complementary to. Consequently, the longer the probe is, the more specific it will be.


What explains why a probe will hybridized to a target gene but not to any unrelated genes?

We design probes to be specific so that they will hybridize only to the target gene but not to random, unrelated genes. A probe hybridizes to a sequences to which it is complementary to. Consequently, the longer the probe is, the more specific it will be.


Which feature of DNA enables a single-stranded DNA probe to hybridize with a single-stranded target gene?

The complementary base pairing between adenine (A) and thymine (T), and cytosine (C) and guanine (G) allows a single-stranded DNA probe to hybridize with a single-stranded target gene. This specificity is key for detecting and identifying specific sequences in the target gene through base pairing interactions.


When a radioactive molecule is attached to a probe a site where the probe bound is its target can be identified because of what?

It Is A


Which explains how a probe can find a single-standard target DNA gene?

The probe is the second strand of DNA that forms double-stranded DNA with the target gene.


What allows a DNA probe to find a single-stranded target gene?

Diploid cells


Why is the hybridization probe said to be complementary?

The hybridization probe is said to be complementary because it is designed to bind specifically to its complementary target sequence in the DNA or RNA of interest. This complementary binding is necessary for the probe to accurately detect and identify the target sequence in a sample.


How can one effectively design an in situ hybridization probe for accurate and specific detection of target sequences?

To design an effective in situ hybridization probe for accurate and specific detection of target sequences, one should consider the following steps: Select a target sequence that is unique to the gene of interest. Design a probe that is complementary to the target sequence and is of appropriate length (usually around 20-30 base pairs). Avoid regions of high sequence similarity with other genes to prevent non-specific binding. Consider the melting temperature (Tm) of the probe to ensure optimal hybridization conditions. Label the probe with a detectable marker, such as a fluorescent dye or enzyme, for visualization. Test the probe for specificity and sensitivity using control samples before conducting the in situ hybridization experiment.


Which of the following determines the specificity of a DNA probe?

complementary base pairing-apex


What is a probe made out of?

a probe is made of medal and other equipment. :}


What property of DNA makes hybridization between a labeled probe and a target gene possible?

The complementary base pairing between DNA strands enables hybridization between a labeled probe and a target gene. The hydrogen bonding between adenine-thymine and guanine-cytosine base pairs allows the probe to specifically bind to its complementary sequence in the target gene, facilitating detection.


What is an atmosphere probe?

An atmosphere probe is a spacecraft or instrument designed to study the atmospheric conditions of a planet or moon. It is typically launched into space and sent to the target celestial body to collect data such as temperature, pressure, composition, and other relevant atmospheric properties.