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How does ethidium bromide interact with double stranded DNA Does it increase or remove supercoiling?
when ethidium ion intercalates between two dna base pairs in a circular dna it causes the dna to unwind by 26 degrees, thereby decreasing twist and increasing writhe. in a circular dna which is negatively supercoiled, if ethidium is added it will become relaxed and if more ethidium is added dna becomes positively supercoiled
What bio molecule present in mitochondria allows it to reproduce independantly of the cell?
DNA is responsible for reproduction.Mitochondria have circular DNA.
What is a circular muscle cell?
A circular muscle cell is a type of muscle cell that is oriented in a circular or transverse direction around a structure. These cells are responsible for contracting and relaxing to control the size of lumens within hollow organs like the intestines or blood vessels.
Does DNA have mitochondria?
No, DNA does not have mitochondria. Mitochondria are structures found within cells that have their own DNA, separate from the DNA found in the cell's nucleus. Mitochondria are responsible for producing energy for the cell.
Which force is responsible for making planets and satellites travel in near circular orbits?
Gravity is the force responsible for making planets and satellites travel in near circular orbits around a central body, such as the Sun or a planet. The gravitational pull between the central body and the orbiting object keeps it in a stable, elliptical path.
What is the visual indication of a supercoiled plasmid on a gel electrophoresis image?
On a gel electrophoresis image, a supercoiled plasmid appears as a tight, condensed band that migrates faster than other forms of the plasmid, such as linear or relaxed circular forms.
Why are two confirmations of plasmids seen when run on a gel when naturally its always negatively super-coiled?
During the isolation of plasmid it is possible that the shearing forces may cause the DNA to break opening the DNA molecule into linear DNA rather than circular supercoiled DNA. The linear DNA moves slower than the circular DNA. Thus two bands might be seen.
How does ethidium bromide interact with double stranded DNA Does it increase or remove supercoiling?
when ethidium ion intercalates between two dna base pairs in a circular dna it causes the dna to unwind by 26 degrees, thereby decreasing twist and increasing writhe. in a circular dna which is negatively supercoiled, if ethidium is added it will become relaxed and if more ethidium is added dna becomes positively supercoiled
What muscles are responsible for pupil construction and dilation?
circular muscles
How do I turn mils into circular mils?
To convert mils to circular mils, you simply square the mil measurement. Since one circular mil is defined as the area of a circle with a diameter of one mil, you can use the formula: Circular mils = (mils)². For example, if you have a wire with a diameter of 10 mils, the conversion to circular mils would be 10², resulting in 100 circular mils.
When an object is moving with uniform circular motion the centripetal acceleration of the object?
The centripetal acceleration of an object in uniform circular motion is directed towards the center of the circular path and is perpendicular to the object's velocity. It is responsible for changing the direction of the object's velocity, keeping it moving in a circular path.
The acceleration that occurs in circular motion?
The acceleration that occurs in circular motion is called centripetal acceleration. It is directed towards the center of the circle and is responsible for keeping an object moving in a circular path. Centripetal acceleration is required because the direction of an object's velocity is constantly changing in circular motion.
When an object is in circular motion which direction is its acceleration?
The acceleration of an object in circular motion is directed towards the center of the circle. This centripetal acceleration is responsible for constantly changing the object's direction, while the object's velocity remains tangent to its circular path.
What when an object moves in a circular path the net force is called a?
Centripal acceloration is the net force when an object moves in a circular path.
What is the function of solution 2 in plasmid isolation?
This solution contains the detergent sodium dodecyl sulfate (SDS) which dissolves the cell membrane and denatures proteins. The solution is very alkaline (pH > 12) due to the presence of sodium hydroxide. The high pH aids in denaturing proteins and causes the cleavage of the phosphate bonds in RNA. This eliminates interference from high molecular weight RNA during the plasmid purification. Under highly alkaline conditions, the two strands in non-supercoiled DNA (linear fragments of chromosomal DNA, relaxed and nicked circular DNA) separate and are partially removed from solution. However, this does not occur with supercoiled forms of plasmid DNA because the two strands are intertwined and entangled in a way that prevents them from coming apart. Therefore, supercoiled plasmid remains free in solution.
What bio molecule present in mitochondria allows it to reproduce independantly of the cell?
DNA is responsible for reproduction.Mitochondria have circular DNA.
What force is needed to keep an object in circular motion?
The centripetal force is the force needed to keep an object in circular motion. This force is directed towards the center of the circular path and is responsible for continuously changing the direction of the object's velocity. It depends on the mass of the object, the speed at which it is moving, and the radius of the circular path.
