Results for: dNTPs

What is the role of magnesium chloride in PCR?

An essential cofactor for the DNA polymerase in PCR is Magnesium chloride. Its concentration must be optimized for every primer:template system. Many components of the reaction bind magnesium ion, including primers, template, PCR products and dNTPs. The main 1:1 binding… Full Answer

Why are there nucleotides A T G and C in the master mix and what are the other components and what are there functions?

The nucleotides also called dNTPs are free nucleotides that are used in the replication of new strands. The complete master mix contains Taq DNA polymerase, deoxynucleotides, oligonucleotide primers, magnesium ions, and buffer.Function:Taq DNA-polymerase: Enzyme is heat-resistant as it can withstand… Full Answer

What is pcr and its application?

PCR or polymerase chain reaction is a method to amplify a fragment of DNA. PCR reaction contains template DNA, primers, dNTPs, polymerase enzyme, buffer and water. The thermocycler manage the heat and time to synthesize DNA (denaturation, annealing and extension)… Full Answer

How is a molecule of DNA is replicated?

The process of DNA replication begins at specific sites in the chromosomes termed origins of replication, requires a primer bearing a free 3'-OH, proceeds specifically in the 5'->3' direction on both strands of DNA concurrently and results in the copying… Full Answer