Results for: ethidium-bromide

What is ethidium bromide?

Ethidium bromide is an intercalating agent that attaches itself between the helix of a DNA. Because the ethidium molecule lights up when illuminated by an ultraviolet light, it is used often in biochemistry laboratories so that fragment of DNA that… Full Answer

Why ethidium bromide is carcinogenic?

The purpose of ethidium bromide is as an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. When exposed to ultraviolet light, it will fluoresce with an… Full Answer

Is agar poisonous?

Agar itself is not poisonous. It is a plant derived substance and used in some cuisines. Agar is often used to analyse DNA. For this purpose it is commonly combined with ethidium bromide which is highly carcinogenic. Full Answer

What are example of mutagens?

A mutagen is anything that changes the genetic material of an organism. The most common examples are X-Rays ( or just about any kind of radiation ), and UV light. A few less common ones are ethidium bromide, and bromine. Full Answer

Why are gels stained?

To develop the position of the proteins or nucleic acids bands. The most common stains for proteins are Coomassie brilliant blue and Amido black (among others), and for nucleic acids is ethidium bromide (this compound form a complex with the… Full Answer

Which toxin causes cell mutation?

several. they are grouped together under the term, mutagens. many mutagens are also carcinogens (causing cancer) and vice versa, though some chemicals belong to one group and not the other. molecular mutagens include ENU, benzene, iazide salts, nitrous acid and… Full Answer

What is the function of EtBr?

Ethidium Bromide is used for visualising DNA. When EtBr binds DNA it will glow pink under UV light. This allows you to take a picture of DNA bands in a gel. The gel is soaked in an EtBr solution and… Full Answer