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Advantages of pour plate method over other methods of bacterial colony?
Wiki User
∙ 11y ago
The purpose of the spread-plate technique is to grow and isolate colonies of bacteria. A sample of bacteria is transferred to the agar plate, an environment that provides nourishment for the bacteria to grow. The bacteria sample is applied to the agar plate which a special streaking technique that dilutes the amount of bacteria in each section of the agar plate continuously. This is because if you just swabbed the bacteria onto the plate with no special technique the colonies would grow very densely together and be difficult to study. The streaking technique gradually dilutes the amount of bacteria in each 'quadrant' of the plate, so the last quadrant should have small, isolated colonies that can be easily studied. The spread plate technique is also used for the eneumeration of aerobic microorganisms from the given sample. This can be done by serial diluting the samples, placing 0.1ml of the diluted sample in the middle of an agar plate and spreading the sample over the surface with a help of an L-rod. After the incubation rhe colonies can be counted.
Wiki User
∙ 15y ago
Wiki User
∙ 9y agoThere a few advantages of using the spread plate method. One of the advantages of using the spread plate method is it is better for isolating the bacteria colonies. It also is helpful in isolating organisms.
Wiki User
∙ 9y agoThe spread plate method is used in microbiology to grow colonies of yeast. It is advantageous compared to the pure culture method because it is easier to isolate specific colonies, however less colonies have a chance of surviving.
Wiki User
∙ 15y ago• Small inoculum size • bulky storage • limited shelf life
Wiki User
∙ 10y agoserial dilution can give the relation b/w the consenteration and the growth of bacteria
Wiki User
∙ 11y agogreen foetus
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