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Q: At what time interval is the enzyme working at its maximum velocity?
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What should be done in order to keep the rate constant over the entire time course?

add more substrate. The rate of the reaction drops when the enzyme no longer has a maximum number of substrate molecules to interact with. Above the maximum substrate concentration, the rate will not be increased by adding more substrate; the enzyme is already working as fast as it can. An enzyme can catalyze a certain number of reactions per second, and if there is not sufficient substrate present for it to work at its maximum velocity, the rate will decrease. Therefore, to keep the enzyme working at its maximum, you must add more substrate.


What happens to the rate of enzyme concentration when you increase substrate concentration?

The rate of enzyme reaction is increased when the substrate concentration is also increased. However, when it reaches the maximum velocity of reaction, the reaction rate remains constant.


True or False Low Km means that an enzyme binds very loosely to a substrate andd consequently results in a high velocity compared to an enzyme with a high Km?

False. A high Km means that an enzyme binds very loosely to a substrate and consequently results in a high velocity compared to an enzyme with a high Km.


What is catalytic constant?

Kcat : First-order rate constant (kcat) reflecting the turnover number of the enzyme, or the number of molecules of substrate converted to product per unit time, when the enzyme is working at maximum efficiency, which called also turnover number. Kcat = Vmax / [E]total (Letian) Kcat : First-order rate constant (kcat) reflecting the turnover number of the enzyme, or the number of molecules of substrate converted to product per unit time, when the enzyme is working at maximum efficiency, which called also turnover number. Kcat = Vmax / [E]total (Letian)


Does freezing a enzyme affect it?

When an enzyme is frozen, it only slows down activity. Unlike boiling an enzyme, it does not stop it from working.


What are the effects of freezing on enzyme activity?

When an enzyme is frozen, it only slows down activity. Unlike boiling an enzyme, it does not stop it from working.


What happens when an enzyme catalyzes a reaction?

The rate of a reactions usually increases when catalyzed by an enzyme. For maximum rate of activity, the enzyme needs optimal conditions.


What 2 factors can affect an enzyme from working properly?

pH and Temperature both impact the enzyme's function.What_factors_affect_enzyme_production


What are the optimum temperature and the optimum pH of an enzyme?

Every enzyme works at its maximum rate at a specific temprature called as optimum temprature for that enzyme. AND all enzymes work at their maximum rate at narrow range of pH, called as optimum pH. A slight increase or decrease in pH causes the retardation in enzyme activity or blocks it completely.


What type factors affect an enzyme from working?

Inhibitors.


What happens if an enzyme stops working?

effects the body functions, overaction of other enzyme results in deterioreting the condition.


Do Temperature of the cytoplasm influence the action of enzymes?

Yes, enzymes have optimal working temperatures that differ from enzyme to enzyme