Want this question answered?
ninta ammada thengakulla annnada matyira thendi pulayadi mona patti kunna ninta thantayodu parayada ninta thantada pengaludsa puril kettaruthannu
ninta uppanta beliya unda
Ni-NTA column is a nickel-nitriloacetic acid column that is used to bind polyhistidine residues in order to purify recombinant proteins. The polyhistidine sequence (six histidines in a row) will bind to nickel, which is in turn bound by the nitroloacetic acid. This immobilizes the recombinant protein in the column for later extraction. Polyhistidine-tagging and Ni-NTA column purification:http://en.wikipedia.org/wiki/Polyhistidine-tagNi-NTA product:http://www.biocompare.com/Articles/ProductReview/825/ProductReview.html