0
Can one use concentrated NiSO4 solution to elute His-tagged Ni-NTA bound protein?
MahavirSinghgp5025
Yes, concentrated NiSO4 solution can be used to elute His-tagged Ni-NTA bound protein as the histidine tag binds to the nickel ions on the column. The elution is typically achieved by using a gradient of increasing NiSO4 concentration, allowing the protein of interest to be selectively released from the column. It is important to optimize the elution conditions to maintain protein stability and avoid nonspecific elution.
Add your answer:
Earn +20 pts
What is catalog retailing?
ninta
Why is it important to make sure file handles are secure and free from embedded foreign bodies or splits?
ninta ammada thengakulla annnada matyira thendi pulayadi mona patti kunna ninta thantayodu parayada ninta thantada pengaludsa puril kettaruthannu
What is NiNTA column?
Ni-NTA column is a nickel-nitriloacetic acid column that is used to bind polyhistidine residues in order to purify recombinant proteins. The polyhistidine sequence (six histidines in a row) will bind to nickel, which is in turn bound by the nitroloacetic acid. This immobilizes the recombinant protein in the column for later extraction. Polyhistidine-tagging and Ni-NTA column purification:http://en.wikipedia.org/wiki/Polyhistidine-tagNi-NTA product:http://www.biocompare.com/Articles/ProductReview/825/ProductReview.html
When a muscle tears what cells or tissues tear?
When a muscle tears, it is the muscle fibers themselves that are damaged. This can involve the tearing of the muscle tissue, as well as potentially causing damage to the surrounding blood vessels and connective tissues.
Does this poem use a rhyme scheme?
AABBCCDDEEFF
