No, not really since it is just for cloning. But their should be enough promoter/sequence to provide antibiotic resistance.
The cloning capacity of pBR322 vector is 1-5kb.
i have no idea wat plasmid,cloning ,and vector means so haha 2 u
The good cloning vector should have small size, high copy number, own origin of replication, restriction sites for many restriction enzymes and selectable markers.
there are two types of vectors cloning vector and expression vectors.
functions as a vector
Expression vectors contain translation start and termination codons. Moreover, it contains promoter region
The cloning capacity of pBR322 vector is 1-5kb.
i have no idea wat plasmid,cloning ,and vector means so haha 2 u
A cloning vector that contains a highlhy active prokaryotic promoter just upstream of a restriction site where the eurkaryotic gene can be inserted in the correct reading frame. Such expression vectors allow the synthesis of many eukaryotic proteins in bacterial cells.
plasmid is the type of the cloning vector. other cloning vectors includes cosmids, bacteriophage, phagemids, artifiical chromosomes. clonong vectors are the carriers of certain traits to be inserted in non coding regions of the DNA.
Gene Cloning is used to clone a gene of interest in a vector called plasmid. The chimeric DNA or rDNA formed by cloning is stable and can be used to propagate and sequence the DNA. producing vector containing inulin gene is an example.
The insert capacity of a cosmid vector is about 35-45 kb.
The good cloning vector should have small size, high copy number, own origin of replication, restriction sites for many restriction enzymes and selectable markers.
Competent cells if i not mistaken
No, the gene cassette is made up of the promoter, transgene and terminator.
there are two types of vectors cloning vector and expression vectors.
We can insert about 5-25 kb sized foreign DNA in a lambda bacteriophage vector.