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Refractive index of oil and water?
Glasses of various types have refractive indices ranging from 1,4 to over 2,0
Why does glycerin have a high refractive index?
The base line for the index of refraction is the amount of directional deviation that is produced when a beam of light travels from the air into another substance. The term "high index" is a relative term. Glycerin actually has a low index of refraction when compared to motor oil. I can't be certain but I believe that the density and opacity of a substance plays a big part in the determination of it's relative index.
Why is 100x microscope lens called oil immersion lens?
Because that lens (it does not HAVE to be a 100x lens - other lens magnifications are also available in oil immersion constructions) is made to be in contact with a drop of special oil which covers the specimen. The oil has the same index of refraction as the front lens glass and that way a different optical construction can be used.
What is the difference between the speed of light in a material and in a vacuum?
The speed of light in a vacuum, called c (celerity) is a constant at 300 000 km/sec. In another material, it will be significantly slower. Perhaps as low as 0.5. These 'high refractive glasses' are of immense value in optics and lanthanum is one of the rare earths that are used. Seldom does the refractive index exceed 2.0 however. The speed of light in a vacuum is taken as 1.0 (by definition), and the refractive index is the (speed of light in vacuo) / (speed of light in the substance). Organic liquids have values greater than 2.0, and are used with cover glasses in microscopy - the 'oil immersion lens' application.
What is the refraction index of oil?
200
What would occur if water were accidentally used in place of immersion oil?
When using the oil immersion objective, the oil has the same refractive index as the glass. So it is like an extension of the lens. Water does not have the same refraction index as glass, so the image would not be as clear.
What is the refractive indices of glass water air and immersion oil?
· The refractive indices of watero Water (0° C) 1.33346Water (100° C) 1.31766Water (20° C) 1.33283· The refractive indices of immersion oil is 1.518· The refractive indices of air is 1.000277· The refractive indices of glass is 1.518
Why immersion oil use in gram staining?
because immersion oil has same refractive index as like lens glass of microscope so it give clear image of object
How do you find the refractive index of groundnut oil?
The refractive index is 1,46-1,46 at at 20 oC.
Refractive index of oil and water?
Glasses of various types have refractive indices ranging from 1,4 to over 2,0
What will happen when high power objective is used with water instead of oil?
When a microscope is used with a very high magnification objective the image can be spoiled by the number of refractions as light goes from one medium to another (glass to air, for example). In order to help this a drop of special oil is placed on the slide cover slip and the bottom of the objective lens dips into the oil. The oil has the same refractive index as the glass used in cover slips and the bottom lens in the objective so there is no refraction as the light passes from glass to oil and back to glass. The objective is designed to give the best image with the oil. The refractive index of water is not the same as that of the oil, so the correction would not be right and the objective would not give the best possible image. I would also be a little wary of using water in case it damages the objective. It may be oil-proof, but that doesn't guarantee it is waterproof.this will lead to poor focusing.
What are oil immersion objective lens for?
because the oil have a refractive index near the glass one so we can work at high power without lossing any details by eliminating the air gap between the the specimen and the objective lens >>>> a.sh (iug)
Why isn't the oil immersion lens used in the hanging-drop procedure?
The purpose to use oil is to make it clear in the microscope. Oil is the quintessential "tool" to make oil immersion magnification possible. Oil has the same refractive index as glass, therefore the light is not bent by the air that would otherwise be in the oil's place.
Why would you use oil on a microscope slide?
Light microscopes commonly need oil for two reasons. The first is that a dab of oil will keep a specimen still on the slide holding it in place and allowing for easier manipulation. The second is that at high magnification, the large change in refractive index from the glass lens, to air, to the slide limits the resolution of the image. With oil between the lens and the sample, higher resolution is achieved.
How fast does light travel through oil?
You need to specify which oil for a more accurate answer. Most oils have a refractive index near 1.47 So 3 * 108 / 1.47 = 2.04081633 * 108 m/s
When would you need to use the oil immersion lens?
Not only does oil immersion increase the microscopic resolution of a specimen, it is also transparent. This allows for optimal microscopy views.
What is the function of the oil used with oil immersion lens?
Oil immersion objective lenses are used to further magnify a specimen. The lens requires more light rays to pass through it since it has more mirrors inside, requiring the use of the oil. The oil functions to refract the light rays towards the center of the lens (the normal line of the specimen). By refracting the light towards the center, more rays of light enter the objective, allowing you to see the specimen in a much higher resolution (higher magnification).
