0
Does total activity increase or decrease during protein purification?
Wiki User
∙ 12y ago
Total Activity almost always decreases during protein purification.
Wiki User
∙ 12y ago
Add your answer:
Earn +20 pts
What are the benefits of protein expression and purification?
Protein expression and purification is when proteins are taken and isolated for their individual benefits and then used in many products such as enzymes and in creams. The benefits are that the products they are used in are more natural and have a better balance with the body.
What is the pH in enzymes activity?
pH is a major determinant of protein structure. The function of a protein is directly related to its structure. For example, pepsin (a proteinase- a protein-digesting enzyme) is functional in the low pH of the stomach because the pH of the stomach provides a suitable environment for proper folding, interactions, and the overall shape of the protein. In the small intestine, where the pH is much higher, it has a significant decrease in activity because this is no longer a favorable environment for the protein.
What are the Reason for enzyme purification?
Answer:on a general basis the purity of an enzyme can be described as the degree of amount of enzyme required to catalyze a specific reactionamount of enzyme being inversely proportional to purity
The PI of protein is 9.24 At what PH MY protein binds to the column and which type of column have to use for purifification?
The PI of protein is 9.24 At what PH MY protein binds to the column and which type of column have to use for purification?
What lack of protein will decrease oxygen supply to body tissues?
hemoglobin
What is fold purification in protein purification?
A measure of how much more pure your protein is after a purification step in comparison to the crude. You can calculate this by dividing the purified steps specific activity by the crude steps specific activity.
How do you decrease your carbohydrates?
I was told my a dietician that I need to decrease my carb intake and increase protein
What does protein expression and purification mean?
Protein expression means to clone compounds. Purification is the cleaning process of those compounds. http://en.wikipedia.org/wiki/Protein
Is protein expression and purification a scientific process?
Yes, you are correct. Protein expression and purification is definitely a good example of scientific process.
How do sympathetic and parasympathetic activity control heart rate during hypobaric hypoxic conditions What is the basic mechanism of action?
Sympathetic activity during hypobaric hypoxic condition would increase while parasympathetic action would decrease. Parasympathetic activity via the vegus nerve would decrease its affects on the AV and SA nodes because of the increase in sympathetic activity. Cyclic AMP from the increase in sympathetic activity blocks Ach from binding muscarinic receptors on the nerves. This blocking of Ach would slow G protein from keeping leak K channels being open and for the G Protein to slow its closing of T-type Ca channels. Increase in sympathetic activity leads to norepinephrine and epinephrine being released. They bind to B1 receptors that activate Gs to activate Adenylate cyclase to activate cAMP that causes pro Kinase to open funny channles and t-type Ca channles causing depolerization.
Why you are adding gst tag to the protein during protein purification?
You have many proteins in a mixture. To identify a single protein you add a GST tag. So, when you are doing any further purification you will end up with your protein of choice.
What has the author Miguel Benitez written?
Miguel Benitez has written: 'Protein purification' -- subject(s): Proteins, Purification
What has the author Robert K Scopes written?
Robert K. Scopes has written: 'Protein purification' -- subject(s): Enzymes, Isolation & purification, Proteins, Purification
What effects do increased temperature have on the activity of salivary amylase?
Increase the temperature too much and this protein will denature and stop working.
What are the benefits of protein expression and purification?
Protein expression and purification is when proteins are taken and isolated for their individual benefits and then used in many products such as enzymes and in creams. The benefits are that the products they are used in are more natural and have a better balance with the body.
What is the pH in enzymes activity?
pH is a major determinant of protein structure. The function of a protein is directly related to its structure. For example, pepsin (a proteinase- a protein-digesting enzyme) is functional in the low pH of the stomach because the pH of the stomach provides a suitable environment for proper folding, interactions, and the overall shape of the protein. In the small intestine, where the pH is much higher, it has a significant decrease in activity because this is no longer a favorable environment for the protein.
What are the Reason for enzyme purification?
Answer:on a general basis the purity of an enzyme can be described as the degree of amount of enzyme required to catalyze a specific reactionamount of enzyme being inversely proportional to purity
