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Flaming the mouth of a test tube creates an air flow. Hot air rises. Therefore dust/particles in the air are less likely to fall into your tube.

There is some controversy as to the value of this action. The hot air will also create eddy currents across the mouth of the tube or flask which may actually increase the opportunity for contamination. If there is some data which supports either view, I would like to see it. In my opinion, the reason behind flaming the mouth of a test tube has not been proven, it is just taken to be dogma.

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13y ago
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Wiki User

13y ago

The purpose of flaming the test tube when putting in or out microbes is to prevent

contamination of its content which could normally comes from the ambient air.

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10y ago

This actually sterilizes the mouth of the test tube. It is a back up to prevent contamination.

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Q: Why reason for flaming the mouth of test tube?
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