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E.coli is gram negative bacteria

it give pink colour

for staining bacteri with gram stain

first make smear then put a drop of crstal violet on smear for one minute

now wash it with distill water and then add a drop of iodine a moderator for 30 sec. and wash it drop by drop with alcohol and then with distill water now add counter stain saffranin for one minute and wash with distill water now look the slide under oil immersion of microscope or 100x

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16y ago

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