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Assume, that you digested 500 mg of the soil in acid, and the final volume of your sample is 25 ml. The solution was measured on ICP-AES and 0.5 mg/L Cu was found. How much Cu the soil contains?

25000 micro liter / 500 mg (or 25 ml / 0.5 g) = 50. This is your dilution factor. Multiply your measured result by this factor

0.5 mg/L Cu * 50 = 25 mg Cu per 1 kg of the soil.

Of course, if you diluted your digested sample during the measurement, this dilution factor also must be taken into account.

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How do you calculate the KD value for a given experiment?

The KD value is calculated by dividing the concentration of a ligand that binds to half of the available binding sites on a protein by the concentration of the ligand. This can be determined experimentally using techniques such as fluorescence spectroscopy or isothermal titration calorimetry.


How can one determine the acid dissociation constant (Ka) of a substance without using the pH value?

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