That is a good question! The answer is simple. You do step by step dilution of the serum with probably normal (isotonic) saline solution and test the same for Widal test. This is how you do quantitative test for many other tests also.
manual test of widal method
The main difference between the Widal slide test antigen and the Widal tube test antigen lies in the method of reaction visualization. In the Widal slide test, the reaction is observed on a glass slide under a microscope, whereas in the Widal tube test, the reaction is seen in small test tubes. Both tests detect antibodies against specific Salmonella antigens, but differ in their setup and visual interpretation.
positive or negative with approximately titre.
What is false positive in widal test
I mean widal test result positive after treatment
Isolated Widal test reading does not have much clinical significance. Rising titre of Widal test done one week apart, in a febrile patient, indicates typhoid fever. You can have such rising titre of Widal test in healthy patient, after dose of typhoid vaccine.
1:80 titre is significant for determination of widal positive.
widal test
To a certain extent yes.The post antibiotics Widal Test will have Low titers of agglutinins.Hence the test may be reported as False Negative.
The blood test for typhoid is called the Widal test. It detects antibodies in the blood against the Salmonella typhi bacteria, which causes typhoid fever.
widal test.
this test is applied for enteric fever that include typhoid and paratyphoid.the pathogen can be recovered from the blood during the first 7-10days of illness. the widal test is a convenient method for detecting the abs produced during salmonella inf. this method has its limitations. many individuals possess a low titre agglutinin that reacts with salmonella ags. hence a rising titre give more significance than a single test value. principal:- abs found in the serum are produced in response to exposure to salmonella organisms and will agglutinate a bacterial suspension of salmonella organisms(non infective) which carry homologus ags.