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That is a good question! The answer is simple. You do step by step dilution of the serum with probably normal (isotonic) saline solution and test the same for Widal test. This is how you do quantitative test for many other tests also.
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What is a manual method?
manual test of widal method
How interpret widal test by slide method?
positive or negative with approximately titre.
What is false positive widal test?
What is false positive in widal test
What is the difference between widal slide test Antigen and widal tube test antigen?
widal slide is easier and it save time but widal tube test is not easy and it takes time.
Will Widal Test after positive resul after treatment in Typhoid?
I mean widal test result positive after treatment
How do you read widal test?
Isolated Widal test reading does not have much clinical significance. Rising titre of Widal test done one week apart, in a febrile patient, indicates typhoid fever. You can have such rising titre of Widal test in healthy patient, after dose of typhoid vaccine.
What significant titer for widal's test?
1:80 titre is significant for determination of widal positive.
Name of the blood test for typhoid?
WIDAL
What are the diagnosis for typhoid fever?
widal test
Do antibiotics affect widal test results?
To a certain extent yes.The post antibiotics Widal Test will have Low titers of agglutinins.Hence the test may be reported as False Negative.
How you do widal test?
Widal test is done by qualitative method or quantitative method. Quantitative method:-Take a set of 8dry clean test tubes. Place 1.9ml saline in tube no.1 and 1ml saline in rest of tubes. Add 0.1ml serum in 1st tube mix well it is dilution of 1:20 Transfer 1ml diluted serum from tube no.1 to tube no. 2 and so on. Through tube no.7 discard 1ml of fluid after mixing. leave 1ml saline in tube no.8. Add one drop of appropriate antigen(use only that antigen suspension which has given a positive reaction in screening test. Mix well and incubate overnight at 37.c Examine for agglutination. Interpretation:- Only a titre above 1:80 should be considered as significant A rising titre (done each week)is considered to be definite evidence of infection.
Interpretation of widal test?
this test is applied for enteric fever that include typhoid and paratyphoid.the pathogen can be recovered from the blood during the first 7-10days of illness. the widal test is a convenient method for detecting the abs produced during salmonella inf. this method has its limitations. many individuals possess a low titre agglutinin that reacts with salmonella ags. hence a rising titre give more significance than a single test value. principal:- abs found in the serum are produced in response to exposure to salmonella organisms and will agglutinate a bacterial suspension of salmonella organisms(non infective) which carry homologus ags.
