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Your best bet is a serial dilution, i.e. get 2 10ml volumetric flasks and take 1ml of your solution to be diluted, and make to 10ml with solvent, mix this, then take 1ml of the resulting 10mls and dilute that to 10ml.

2x 10 times dilutions is 100x dilution.

Alternatively if you have less to start with, use micro pipettes

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10y ago
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14y ago

taking 1ml of sample and 99 ml of solution

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7y ago

A 10X solution is known that contains ten times more solute in solution. In order to make a 10X solution, just multiply by 10 the amount of solute(s) to be dissolved.

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Q: How do you prepare 1 is to 100 dilution in small volume?
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