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The principle is based on equilibrium of the molecules between the mobile phase and the stationary phase. This will vary depending on the polarity of the substance, and the two phases. If the substance in more soluble in the mobile phase, it will travel further, and vice versa.

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Procedures that is used to separate different types of molecules in a liquid mixture?

Chromatography is a technique used to separate different types of molecules based on their size, charge, or affinity for a stationary phase in the mixture. This can be done using methods like paper chromatography, thin layer chromatography, or high-performance liquid chromatography (HPLC) depending on the specific separation requirements of the molecules.


What are the key differences between cation exchange chromatography and anion exchange chromatography?

Cation exchange chromatography separates molecules based on their positive charge, while anion exchange chromatography separates molecules based on their negative charge. The key difference lies in the type of charge that is used to separate the molecules.


How do you separate dye to blue ink?

To separate dye into blue ink, you would need to use a process called chromatography. This involves dissolving the ink in a solvent and allowing it to separate based on the different properties of the dye molecules. The blue dye will travel at different rates along the chromatography paper, allowing you to isolate it as a distinct color.


Can chromatography be used to separate mixtures?

Yes, chromatography can be used to separate mixtures into individual components based on their different speeds of migration through a stationary phase. The components of the mixture will separate based on their differing affinities for the stationary phase.


What type of substance was chromatography originally designed to separate?

Chromatography was originally designed to separate plant pigments, particularly chlorophyll, from a mixture.


What are the key differences between anion exchange chromatography and cation exchange chromatography?

Anion exchange chromatography and cation exchange chromatography are both types of ion exchange chromatography used to separate molecules based on their charge. The key difference between them is the type of ions they attract and retain. Anion exchange chromatography attracts and retains negatively charged ions (anions), while cation exchange chromatography attracts and retains positively charged ions (cations).


How will you separate the components of black ink using chromatography?

You can use the filter paper or chromatography paper to separate the different components in black ink.


What is the difference between anion and cation exchange chromatography?

Anion exchange chromatography separates molecules based on their negative charge, while cation exchange chromatography separates molecules based on their positive charge.


What is the difference between cation and anion exchange chromatography?

Cation exchange chromatography separates molecules based on their positive charge, while anion exchange chromatography separates molecules based on their negative charge.


What is a sentence for chromatography?

The chemist will now separate the complex mixture by utilizing a method known as chromatography.


How far do polar molecules travel in thin-layer chromatography (TLC)?

Polar molecules travel a shorter distance in thin-layer chromatography (TLC) compared to non-polar molecules.


If you have a mixture of substances with different - sized molecules. How could you separate them?

One way to separate substances with different-sized molecules is through a process called chromatography, where the mixture is passed through a material that selectively interacts with the molecules based on their size. Another method is fractional distillation, where the mixture is heated to separate the components based on their boiling points. Size exclusion chromatography is also effective, as it separates molecules based on their size by allowing smaller molecules to travel through the column more slowly than larger ones.