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Resolution is the ability of a lens to separate or distinguish between small objects that are close together. Much of the optical theory underlying microscope design was developed by the German physicist Ernst Abbe in the 1870s. The minimum distance (d) between two objects that reveals them as separate entities is given by the Abbe equation, in which lambda () is the wavelength of light used to illuminate the specimen and n sin theta is the numerical aperture (NA). d= .5 x lambda n sin theta

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โˆ™ 2008-08-19 13:09:35
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Q: How is the formula for resolving power of a microscope derived?
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Related Questions

What is the Resolving power of light microscope?

the resolving power of light microscope is 0.2 micrometr


What is the resolving power of a transmission electron microscope?

The resolving power of the transmission electron microscope is 100 times better than the resolving power of the scanning electron microscope. The resolving power of the compound light microscope is 1000 times better than the resolving power of the unaided eye.


How do you calculate resolving power?

Resolving power is a function of numerical aperture of the objective lens use in the microscope and the wavelength of light . The formula for resolving power is given as , RP= wavelength / (2 x NA) where NA is the numerical aperture.


Explain the relationship between the resolving power of the microscope and the wavelength of the light being used?

Resolving power of microscope is inversely related to the wavelength of the light used. So shorter the wavelength, greater the resolving power.


What is the difference between the magnifying power and the resolving power of a microscope?

The magnifying power of a microscope is how big the microscope can make the image and how close it can make the image to the eye. Resolution or resolving power is how clear the microscope can focus the object and how much detail is provided in the magnification.


What is the resolving power of a compound microscope?

.2 um


Which has the least resolving power?

A transmission electron microscope.


Why does an electron microscope have a greater resolving power than a light microscope?

The resolving power of a microscope is a linear function of the wavelength - An optical microscope's wavelength is that of light, and the electron microscope's - that of vibrating electrons. As the electron microscope's wavelength is about 100,000 times smaller than that of light, we get a much better resolving power.


What is the Resolving power formula?

The question is about the resolving power of optical instruments like telescope and microscope.It is the ability of the instrument to resolve the images of two points that are close to each other. If dθ is the angular separation, resolving power is given by the formulaR = 1/dθ = D/1.22 λ where Dis the aperture of the objective; λ is the wavelength of the light .


What limits the resolving power of an electron microscope?

The wavelength of the electron.


What happens to contrast and resolving power when the aperture of the condenser of a compound microscope is decreased?

Contrast increases, while resolving power decreases.


What is the resolving power of an electron microscope?

Resolving power is the ability of an imaging device to separate points of an object that are located at a small angular distance


What is resolving power and why is it important in biology?

resolving power is the ability of an imaging device to separate distinctive points of an object; it is important because the more resolving power a microscope has the better we can see the cell and its structures


What is the maximum resolving power of a compound light microscope?

0.2 micrometer


The ability of a microscope to reveal fine detail is known as?

Resolving Power


What is the importance of resolving power in microscopy?

The resolving power of a microscope is important because it is the limit of what structures can be imaged and observed. Structures and features smaller than the resolving power will not be clearly viewed, under any circumstances.


Power of microscope?

Depends which type of microscope we are talking about. The common compund light microscope has a resolving power of 0.2 micrometer or 0.0002 millimeter. In comparison the human eye's resolving power is 0.1 millimeter. Resolving power is the minimum distance between two objects or particles such that the objects are distinguishable. So for example in the case of human eyes with resolving power of 0.1 millimeter, if you bring two objects any closer to each other than 0.1 mm, our eye cannot tell if they are two separate objects or not. Last but not least, the lower the resolving power, the higher the resolution. So because a compound microscope has a lower resolving power than human eye, it has a higher resolution.


What is the maximum resolving power of a light microscope?

Around 0.2micrometers or 200 nm


Is the magnification of a light microscope limited to 1000X by the resolving power of the instrument?

True!


What limits the amount of magnification that can be produced using light?

The resolving power of the microscope.


Why can ribosomes be seen with an electron microscope but not a light microscope?

electron microscopes have more resolving power than light microscopes


What limits the resolving power of a light microscope?

wavelength of light used and the diffraction effect.


What does resolving power of a microscope mean?

its ability to distinguish two items at its highest magnification


Why true solutions are not visible with microscope even with electron microscope?

Electron microscopes don't have the resolving power to see individual molecules.


What happens to contrast and resolving power when the aperture of the condenser of a compound light microscope is decreased?

It is decreased along with the amount of light that is decreased