Total magnification is determined by multiplying the magnification of the ocular lens by that of the objective lens. Compound microscope that uses more than one lens to direct light through a specimen mounted on a glass slide.
The multiplication of the eyepiece lens (usually 10x) times the multiplication of the objective lens (usually 4x, 10x, 40x and 100x)
So total magnification would be 40x (4x times 10x), 100x , 400x and 1000x.
Multiply the eyepiece magnification times the objective magnification.
1000X magnification
Yes, the wavelength of the light limits the maximum magnification of a microscope. Using visible light, the limit is about 1200 to 1500X.
The total magnification would be 500x...you take the ocular and multiply it by whatever objective you are using.
400x
50 micrometers
It's actually simple: The more the magnification, the smaller a "picture" of the available light you are getting - hence the more magnification, the darker the image looks. It works the same way with an astronomical telescope: The more magnification you use (assuming the objective lens doesn't change) the dimmer the object gets. To put it another way - there is a big difference between how high a magnification you are using, and how much light is reaching your eye.
magnification
The total magnification would be 200x, since the total magnification is the magnification of the objective lens X the magnification of the eyepiece.
1000X magnification
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Yes, the wavelength of the light limits the maximum magnification of a microscope. Using visible light, the limit is about 1200 to 1500X.
Multiply the magnification of the eyepiece (usually 10x) and the magnification of the objective you are using, Example: eyepiece = 10 x objective lense = 40x 10 x 40 = 400 magnification of 400x.
First you must obtain an adequate sample of the cells (and their nucleus) which you wish to measure. Once you have the slides you have to methods of measuring: 1.- using a microscope with a graded eyepiece. This requires a few simple steps of calculation regarding magnification of the different lenses on the microscope. 2.- Capturing digital images of the cell nucleus at a known magrnification and then using a program to measure the diameter of the cell nucleus ( I am currently using the free program IMAGETOOLS to do exactly that!!) This also requires a few simple steps of calculation regarding magnification of the different lenses on the microscope.This requires a few simple steps of calculation regarding magnification of the different lenses on the microscope....but in this case the computer program does it for you.
The total magnification would be 500x...you take the ocular and multiply it by whatever objective you are using.
it has light limited magnification
The total magnification of the microscope when using the 40x objective depends on the strength of the eye piece lens. Typically a 10x eye piece lens is used in college microscopes this would give 40x10 = 400x magnification.
450x TM ( magnification of the ocular lens ( 10x) multiplied by the magnification from the objective lens ( 45x)= 450x TM ( total magnification)