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It has happened in as little as a single generation in the case of orchids. Generally speaking, groups of organisms that do not interbreed on on the borders of speciation, whereas those that cannot interbreed are considered to be separate species.

Dromedaries and llamas have been separated by more than ten million years, yet they can interbreed. Humans and chimpanzees are separated by less than six million years, yet it is doubtful our species can interbreed.

It probably depends significantly on the amount of change between groups, but I would estimate most species separated for more than a hundred thousand years would be sexually incompatible.

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Q: How long does divergent evolution take to occur?
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