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To make a dilution of guanidine isothiocyanate (GITC), first prepare a stock solution at the desired concentration by dissolving the solid in a suitable buffer or water. Then, to dilute this stock solution, mix a specific volume of the stock with an appropriate volume of diluent, such as phosphate-buffered saline (PBS) or water, following the dilution formula (C1V1 = C2V2), where C1 is the initial concentration, V1 is the volume of the stock solution used, C2 is the final concentration, and V2 is the final volume. Ensure to handle GITC in a fume hood and wear appropriate personal protective equipment, as it is toxic. Always label the final solution with the concentration and date prepared.
What else can I help you with?
What is guanidine salt?
Guanidine is a base. The chemical formula is HNC(NH2)2.
How much solution to 1 liter water 110 dilution?
You add 9.09ml of stock solution to a volumetric and make it up to 1 litre to get a 110 dilution
How much water to add to make a 500 dilution?
To make a 500 dilution, add 1 part of the substance you are diluting to 499 parts of water. For example, if you have 1 mL of the substance, you would add 499 mL of water to make a total volume of 500 mL for the dilution.
Does dilution make an acid weaker?
Dilution of acid does not change its chemical properties, so the acid itself is not weaker. However, dilution can reduce the concentration of the acid in a solution, making it less corrosive or less likely to cause harm upon contact.
What dilution to make 50percent acetone control?
To make a 50% acetone control, you can mix equal parts of acetone and water. For example, if you start with 10 ml of acetone, you would add 10 ml of water to make a 50% acetone solution.
What is the use of guanidine isothiocyanate and NaOAc in the isolation of RNA?
Guanidine isothiocyanate helps denature proteins from the RNA to allow them to be separated from protein for the best isolation of nucleic acids from proteins (can collect all 3 if using TRIzol like reagents)NAoAc (sodium acetate) usually in 3M/pH8 is used later in the steps for nucleic acid isolation as the salt for ethanol precipitation. If you are going to be doing RNA transcription off of DNA templates that you are precipitating, it is best to use Nh4oAC (ammonium acetate) as the ion is nicer to RNA polymerases once templates are cleaned and being transcribed.
What is guanidine salt?
Guanidine is a base. The chemical formula is HNC(NH2)2.
How do you prepare 6M Guanidine HCl?
To prepare a 6M Guanidine HCl solution, weigh out the appropriate amount of Guanidine HCl powder based on the desired volume and molarity. Dissolve the Guanidine HCl in deionized water while stirring continuously until fully dissolved. Adjust the final volume with more deionized water if needed.
What is the mass percentage of nitrogen in guanidine?
The molar mass of guanidine is 59.07 g/mol (C(NH2)3). Nitrogen contributes 42.04 g/mol to this molar mass. Therefore, the mass percentage of nitrogen in guanidine is approximately 71.2%.
What has the author Nikolai A Sivov written?
Nikolai A Sivov has written: 'Biocide guanidine containing polymers' -- subject(s): Polymerization, Guanidine, Polyelectrolytes, Synthesis
Why guanidine is a strong base?
Guanidine is a strong base because it readily accepts protons (H+) to form guanidinium ions. The lone pair on the nitrogen atom of guanidine can easily abstract a proton from a solution, making it a strong base with high basicity.
which amino acids has a guanidine group?
Gasoline.
What is a biguanide?
A biguanide is a compound imidodicarbonimidic diamide derived from guanidine.
What is biguanide?
A biguanide is a compound imidodicarbonimidic diamide derived from guanidine.
Why guanidine is stronger base than ethanamidine?
Guanidine is a stronger base than ethanamidine because it has three nitrogen atoms in its structure, providing more opportunities to accept protons and increase electron density. This electron density makes guanidine more basic compared to ethanamidine, which has only one nitrogen atom for proton donation.
Definition of dilution and dilution factor?
In chemistry and biology, the dilution factor is the total number of unit volumes in which the material is dissolved. As I understand it, the dilution refers to the dilution ratio. If you add 1 part of something to 4 parts of something else, the dilution ratio is 1 to 4. The dilution factor counts all the parts and expresses the same thing as 1 out of 5.
How much solution to 1 liter water 110 dilution?
You add 9.09ml of stock solution to a volumetric and make it up to 1 litre to get a 110 dilution
