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Q: How make a dilution of guanidine isothiocyanate?
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What is the use of guanidine isothiocyanate and NaOAc in the isolation of RNA?

Guanidine isothiocyanate helps denature proteins from the RNA to allow them to be separated from protein for the best isolation of nucleic acids from proteins (can collect all 3 if using TRIzol like reagents)NAoAc (sodium acetate) usually in 3M/pH8 is used later in the steps for nucleic acid isolation as the salt for ethanol precipitation. If you are going to be doing RNA transcription off of DNA templates that you are precipitating, it is best to use Nh4oAC (ammonium acetate) as the ion is nicer to RNA polymerases once templates are cleaned and being transcribed.


What is guanidine salt?

Guanidine is a base. The chemical formula is HNC(NH2)2.


What dilution to make 50percent acetone control?

2 times dilution from 100% acetone


What is the mass percentage of nitrogen in guanidine?

71%


What has the author Nikolai A Sivov written?

Nikolai A Sivov has written: 'Biocide guanidine containing polymers' -- subject(s): Polymerization, Guanidine, Polyelectrolytes, Synthesis


Does dilution make an acid weaker?

Yes


which amino acids has a guanidine group?

Gasoline.


Why guanidine is a strong base?

due to the imine bond


What is biguanide?

A biguanide is a compound imidodicarbonimidic diamide derived from guanidine.


What is a biguanide?

A biguanide is a compound imidodicarbonimidic diamide derived from guanidine.


Definition of dilution and dilution factor?

In chemistry and biology, the dilution factor is the total number of unit volumes in which the material is dissolved. As I understand it, the dilution refers to the dilution ratio. If you add 1 part of something to 4 parts of something else, the dilution ratio is 1 to 4. The dilution factor counts all the parts and expresses the same thing as 1 out of 5.


If it takes 11 ounces of chemical in 10 gallons of water to make a finished dilution of 05 How many ounces of chemical would it take to make 100 gallons of finished dilution at 05?

110