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Q: How many multiple cloning sites are in pbr322?
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What is universal primer?

Universal primers are really not 'universal' in the sense that they will bind to anything. Universal is kind of a misnomer. Really, universal primers are PCR/sequencing primers that bind to a sequence found in many plasmid cloning vectors, most of which are derived from pUC vectors (which in turn come from pBR322). These sequences were defined as good PCR and sequencing sites as they flank the multiple cloning site where an inserted DNA sequence would be put. You can now buy these universal primers from various companies. You can see that these primers are called universal because they can be used to amplify or sequence any insert that is put in the multiple cloning site.


How many bands were produced after pBR322 was digested with EcoR1?

pBR322 has one EcroR1 site so 1 band however if it was not fully digested you will find 2 or 3 (Linear- [cut], Supercoiled-, Round-Plasmid).


What a good cloning vector should have?

The good cloning vector should have small size, high copy number, own origin of replication, restriction sites for many restriction enzymes and selectable markers.


How many sections are there in cloning?

what are the sections are there in cloning


How many ways of cloning are there?

their are many ways cloning like 20 ways


Online site for computer servers.?

There are many different online sites where you can purchase multiple computer servers. One of the best-quality providing sites I have found is itcomputerservers.com


Is cloning morally right why?

This is an ethical question and is based on personal opinions.There are many types of cloning and two of them are reproductive and therapeutic. Usually when people think that cloning is ethically right, they are mostly focusing on therapeutic cloning which is only creating a whole new single body part from a single cell. People who generally think that cloning is wrong are focusing more on the reproductive cloning which creates an entirely new organism.Both sides have their pros and cons. For example, therapeutic can create new body parts for a sick person and save their lives, using the same person's cells. But reproductive cloning may cause many problems such as people have too similar DNA and letting new diseases arise.My personal opinion is that cloning should be allowed for therapeutic reasons only but should be controlled so the research does not get out of control. If people were able to learn how to clone someone or something, this could cause multiple issues for humans and other species.


What are the ethical issues with cloning cattle embryos?

There are not many ethical issues with embryo cloning in cattle but one of them is that it's not natural.


Where can one get a machine for making ID badges?

You can get these machines from many sites. One of these sites is AlphaCard where they offer multiple types of ID Card making machines starting from $875


How many people died from doing cloning?

No one.


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There are many different websites that offer vacation packages. You could try KAYAK.com, as they will allow you to search multiple travel sites at once.


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There are many ways to get car rental quotes. You can visit a company's site directly (i.e. www.enterprise.com) or check one of the many price matching sites that will return multiple quotes (i.e. www.priceline.com).