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The good cloning vector should have small size, high copy number, own origin of replication, restriction sites for many restriction enzymes and selectable markers.
their are many ways cloning like 20 ways
Many people have a personal opinion on cloning. Most people do not think it is a good idea and there has even been a bill known as the Human Cloning prohibition Act made to Congress but was rejected. It was re-introduced again in May 2013.
Human cloning is not possible. Many cloning attempts on our closest relatives, rhesus monkeys, have resulted in utter failure with not a single successful birth. Cloning in cattle have also resulted in very few pregnancies and even fewer are born. Most of those born suffer from bizarre illnesses that never occur in nature.
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Universal primers are really not 'universal' in the sense that they will bind to anything. Universal is kind of a misnomer. Really, universal primers are PCR/sequencing primers that bind to a sequence found in many plasmid cloning vectors, most of which are derived from pUC vectors (which in turn come from pBR322). These sequences were defined as good PCR and sequencing sites as they flank the multiple cloning site where an inserted DNA sequence would be put. You can now buy these universal primers from various companies. You can see that these primers are called universal because they can be used to amplify or sequence any insert that is put in the multiple cloning site.
pBR322 has one EcroR1 site so 1 band however if it was not fully digested you will find 2 or 3 (Linear- [cut], Supercoiled-, Round-Plasmid).
The good cloning vector should have small size, high copy number, own origin of replication, restriction sites for many restriction enzymes and selectable markers.
what are the sections are there in cloning
their are many ways cloning like 20 ways
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This is an ethical question and is based on personal opinions.There are many types of cloning and two of them are reproductive and therapeutic. Usually when people think that cloning is ethically right, they are mostly focusing on therapeutic cloning which is only creating a whole new single body part from a single cell. People who generally think that cloning is wrong are focusing more on the reproductive cloning which creates an entirely new organism.Both sides have their pros and cons. For example, therapeutic can create new body parts for a sick person and save their lives, using the same person's cells. But reproductive cloning may cause many problems such as people have too similar DNA and letting new diseases arise.My personal opinion is that cloning should be allowed for therapeutic reasons only but should be controlled so the research does not get out of control. If people were able to learn how to clone someone or something, this could cause multiple issues for humans and other species.
There are not many ethical issues with embryo cloning in cattle but one of them is that it's not natural.
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No one.
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