the primary transcript usually has a exons and introns which need to undergo splicing to remove the introns and re-splicing to join the exons ..after this process the resulting mRNA is a mature mRNA.
The introns are the sections which are spliced out to create the mature form of mRNA.
The mRNA produced by transcription is similar to a rough cut of a film that needs a bit of editing. A specialized nucleotide is added to the beggining of each mRNA molecule, which forms a cap. It helps the mRNA starand bind to a ribosome and prevents the strand from being broken down to fast. The end of the mRNA molecule gets a string of A nucleotides, called the tail, that helps the mRNA molecule exit the nucleus.
This is sometimes referred to as the Primary Dogma of Biology:The DNA code is transcribed to produce pre-messenger RNA (mRNA) which is refined into mature mRNA and transported out of the nucleus. The mRNA is then translated into an amino acid chain by a ribosome. The amino acid chain is then refined into a protein.
In eukaryotic organisms, genes consist of exons and introns. Exons are regions that are transcribed into mature messenger RNA, and eventually translated into protein. Interspersed within the exons are introns, regions of non-coding DNA. Introns must be removed from the initial transcript of mRNA before the final mature transcript is sent to the ribosome for translation into protein. This removal is done in a coimplex protein structure called the spliceosome. The spliceosome splices out the non-coding introns from the primary mRNA transcript, and stiches the exons back together into the mature mRNA transcript.
RNA Polymerase
From the nucleus where it is synthesized, the mRNA passes into the cytoplasm and become attached to ribosomes.
The first (primary) transcript from a protein coding gene is often called a pre-mRNA and contains both introns and exons. Pre-mRNA requires splicing (removal) of introns to produce the final mRNA molecule containing only exons
A primary RNA transcript may contain as many as 200,000 nucleotides (the average for human cells is 5,000). Yet mRNA in the cytoplasm averages only 1,000 nucleotides. All three types of RNA are processed in the nucleus of eukaryotes before they leave the nucleus. Enzymes add additional nucleotides and chemically modify or remove others. Mature RNA contains
Exons code for proteins. Exons are parts of DNA that are converted into mature messenger RNA.. From there, mRNA undergoes translation where mRNA is used to synthesize proteins.
The excising of the introns from the mRNA and the adding of the poly A tail and guanine repeats to the mature mRNA so that it now exits the nucleus to begin translation.
The excising of the introns from the mRNA and the adding of the poly A tail and guanine repeats to the mature mRNA so that it now exits the nucleus to begin translation.