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The transformants are selected for on agar containing an appropriate antibiotic. For example if your recombinant plasmid contains a kanamycin cassette, then only the cells containing the plasmid will grow on an agar plate containing kanamycin. PCR can then be performed on the colonies to ensure they contain your gene of interest on the plasmid.
Wiki User
∙ 15y ago
Wiki User
∙ 12y agoThe plasmid must include a genetic marker that allows for selection of recombinant cells. This marker is usually antibiotic resistance, normally ampicillin in bacteria and Geneticin in mammalian cell lines. After treating the cells with the appropriate antibiotic, only the cells that have the vector will have survived.
In short, they aren't identified, they're selected for.
Wiki User
∙ 13y agoby using a radioactive marker, usually antibiotic resistant
Wiki User
∙ 14y agoseeing it with your eyes..
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A population of cells carrying a desired plasmid is called a?
Clone
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmis into a bacterium?
I. Transform bacteria with recombinant DNA molecule II. Cut the plasmid DNA using restriction enzymes III. Extract plasmid DNA from bacterial cells IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments V. Use ligase to seal plasmid DNA to nonplasmid DNA
What is a small circular molecule of bacterial DNA that can be used to transfer DNA into a living cell?
It's called a plasmid, but it can't be used for eukaryotic cells, only prokaryotic (bacteria). It's the basis of recombinant molecular biology.
How is recombinant DNA transffered?
Recombinant DNA can be transformed (you meant transferred) in to the bacterium by heat shock treatment or by electroporation. Cloned rDNA is normally mixed with the competent cells prior to this. Transformation cause the entry of the plasmid DNA into bacterium. They cultured for a while and plated against antibiotics to isolate transformants.
What type of gene is used to distinguish bacteria that carry a plasmid containing foreign DNA from those that don' t?
The plasmid that contains foreign DNA is engineered to also carry an antibiotic resistance gene. This antibiotic resistance gene codes for a protein that is able to inactivate an antibiotic thus keeping the cell alive. In the absence of the antibiotic resistance gene, the cells would not survive when exposed to an antibiotic. After transfection (the process of inserting the plasmid carrying the foreign gene into cells), the cells are gown in media containing an antibiotic. Cells that contain the plasmid (and therefore contain the antibiotic resistance gene) are able to survive in this medium. Cells that do not contain the plasmid (and therefore lack the antibiotic resistance gene) do not survive in this medium. The process described above is called selection
What do you call a population of cells carrying a desired plasmid?
A plasmid is a small molecule of DNA that replicate independently within the cell. A population of cells carrying a desired plasmid is called a clone.
A population of cells carrying a desired plasmid is called a?
Clone
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmis into a bacterium?
I. Transform bacteria with recombinant DNA molecule II. Cut the plasmid DNA using restriction enzymes III. Extract plasmid DNA from bacterial cells IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments V. Use ligase to seal plasmid DNA to nonplasmid DNA
Are plasmid vectors helpful?
Plasmid vectors are an invaluable genetic engineering tool for inserting recombinant DNA sequences into different organisms or cells in culture.Plasmids are essentially circular DNA constructs composed of some essential elements like:An origin of replicationA multiple cloning site which consists of restriction sites where the recombinant DNA can be insertedMarker genes (like antibiotic resistance)reporter genes to confirm a successful transformation
What is a small circular molecule of bacterial DNA that can be used to transfer DNA into a living cell?
It's called a plasmid, but it can't be used for eukaryotic cells, only prokaryotic (bacteria). It's the basis of recombinant molecular biology.
Compare and contrast animanl and plant cells?
In bacteria, if the plasmid containing the foreign DNA manages to get inside a bacterial cell, this sequence ensures that it will be replicated. In Plant Cells, if transformation is successful the recombinant DNA is integrated into one of the chromosomes of the cell.
Explain how DNA technology transfer bacterial genes from cell to cell?
I think you must rethink about your question, but still I am giving the answer as I can understand that you are asking about recombinant DNA technology where bacterial DNA is used as it is a cloning vector (plasmid). In recombinant DNA technology the particular sequence of DNA that we want to replicate or want to produce in huge number, is attached either with plasmid of bacteria or a DNA of bacteriophage and thus produce the recombinant or hybrid DNA which is copied each time when the bacteria or bacteriophage multiply. In this way the hybrid DNA will be transferred from parent cell to daughter cells.
How is plasmid DNA similar to the DNA found in chromosomes?
The plasmid DNA is a small self replicating particles other than the DNA found in the chromosomes. The both DNA are self replicating but the plasmid DNA is very important in techniques like nucleic acid hybridizations , radio graph micro assay, PCR and many other. Both of the DNA are used in recombinant technology.
How do you identify the water carrying cells in a stalk of celery?
They are a different shape and have a dark premiter!
What is a plasmid in a cell?
A circular, double-stranded unit of DNA that replicates within a cell independently of the chromosomal DNA. Plasmids are most often found in bacteria and are used in recombinant DNA plasmidto transfer genes between cells.
How is recombinant DNA transffered?
Recombinant DNA can be transformed (you meant transferred) in to the bacterium by heat shock treatment or by electroporation. Cloned rDNA is normally mixed with the competent cells prior to this. Transformation cause the entry of the plasmid DNA into bacterium. They cultured for a while and plated against antibiotics to isolate transformants.
What is found in only prokaryotic cells?
plasmid
