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How you differentiate between Transformed bacterial colonies and satellite colonies?
Re-streak the center of the 'star' colony (transformed surrounded by satellites) on a plate contains the antibiotic, typically ampicillin. The colonies in the tertiary streak will most likely be the transformants. If you want to be quite sure, pick a single colony from the tertiary streak and re-streak again on a plate containing the antibiotic.
What else can I help you with?
What was true about the American colonies after the Continental Congress met?
The American Revolution transformed thirteen British colonies into fourteen states.
In what ways do the macroscopic features of bacterial colonies differ from that of molds?
Bacteria looks more glossy, white or yellow Molds will have a fuzzy look to them
Why then are bacterial colonies still able to adapt to new environments?
Bacterial colonies can adapt to new environments due to their high mutation rates and genetic diversity, which allow for rapid evolution. Additionally, mechanisms such as horizontal gene transfer enable bacteria to acquire beneficial traits from other organisms, enhancing their ability to survive in changing conditions. Their short generation times facilitate quick adaptations to environmental pressures, making them highly resilient and versatile.
How do you distinguish between bacterial colonies and yeast colonies?
Under a microscope a yesat should have long filamentous hyphae whereas bacteria are simple rods or cocci. Acidic or antibacterial mediums should kill most bacteria most fungi (including yeasts) will remain living.
What are colonies of bacteria?
Bacterial colonies are defined as the assamblage of bacteria growing on a solid surface such as the surface of an agar culture medium, the assemblage often is directly visible, but also may be seen only microscopically.
Do yeast colonies resemble bacterial or fungal colonies?
bacterial
What does the phenotype of the transformed colonies tell you?
The phenotype of the transformed colonies can reveal information about the characteristics conferred by the introduced foreign DNA. It may indicate traits such as antibiotic resistance, fluorescent protein production, or enzyme activity, depending on the gene that was inserted. This can help confirm successful transformation and expression of the desired gene in the bacterial colonies.
What is non selective media?
Non-selective is when we use agar for bacterial growth in general. Mean not to differentiate between them but just to see their colonies and growth. TSA and Chocolate agar are nonselective.
What are the problems with agar plates?
Agar plates can become contaminated with unwanted microorganisms, leading to inaccurate results. They can also dry out, affecting the growth of bacteria. Additionally, agar plates can become overcrowded with bacterial colonies, making it difficult to differentiate and count individual colonies.
Yeast colonies larger than bacterial colonies?
Not necessarily, some Bacillus can have big colonies
How were the colonies transformed into sovereign states?
(by forming their own governments)
Why GFP colonies are observed in the plate with pQE30 and GFP construct?
Go to http://www.tsienlab.ucsd.edu/Images.htm you will find bacterial colonies will and can express functional fluorescent proteins. As for the plasmid pQE30, it looks to fit into the category of expression at this level.
Are satellite colonies sensitive to ampicillin?
Yes, satellite colonies may be observed radiating from the edges of large, well-established colonies. These satellite colonies are not ampicillin-resistant, but grow in an "antibiotic shadow", where ampicillin in the media has been broken down by the large resistant colony. Satellite colonies are generally a sign of antibiotic weakened by not cooling medium enough before adding antibiotic, long-term storage of more than 30 days, or overincubation.
How to determine Bacterial load?
By pour plate and then counting the colonies.
How many bacterial colonies are in a guinea pig mouth?
a lot
Do all bacterial colonies consist of pure cultures?
YES.!!
What was true about the American colonies after the Continental Congress met?
The American Revolution transformed thirteen British colonies into fourteen states.
