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Does Mycobacterium phlei form capsules?
No, Mycobacterium phlei is not known to form capsules. Capsule formation is more commonly associated with certain pathogenic bacteria to help evade the host immune response. Mycobacterium phlei is a non-pathogenic soil bacteria and does not typically form capsules.
Does Mycobacterium phlei grow on EMB agar?
Mycobacterium phlei is a non-fastidious organism that is commonly found in soil and water. It is not commonly grown on Eosin Methylene Blue (EMB) agar, as EMB agar is more selective for gram-negative enteric bacteria. Instead, Mycobacterium phlei is typically cultured on media specifically designed for mycobacteria, such as Middlebrook agar or Lowenstein-Jensen agar.
Is mycobacterium phlei gram positive or negative?
Although mycoplasma lack a cell wall and therefore test gram negative, they are considered to be descendents of 'nonsporulating and endospore forming gram-positive bacteria' (Madigan et al., 2009), such as Lactobacilli, Bacilli and Streptococci, (Dandekar et al., 2002), which have lost their cell wall. Mycoplasmas are therfore classed as gram- positive bacteria. This is supported by ribosomal RNA and DNA analysis.
