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A antibody or immunization is the ultimate goal. Sub-cloning resulted in GST-N fusion protein purified by chromatography and used as antigen for serological tests. For example, in studies two anti-N specific monoclonal antibodies (MAbs) (IAF-K8 and IAF-2B4), obtained following fusion experiments with spleen cells of BAlb/c mice that were immunized with the purified virus.
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∙ 9y ago
To subclone and express the nucleocapsid protein in E. coli, the nucleocapsid gene is first amplified by PCR and then inserted into an expression vector, typically one that contains a suitable promoter for expression in E. coli. The recombinant vector is then transformed into E. coli cells, which will produce the nucleocapsid protein when induced by suitable growth conditions. The expressed protein can be purified from the cell lysate using techniques such as affinity chromatography.
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What is E. coli bl21?
an E. coli B strain, usually used for protein-expression.
How can you get membrane protein from E. coli?
What is inner membrane protein and outer membrane protein How can you get membrane protein from Ecoli and how to separate the inner and outer membrane protein.
What is inner membrane protein and outer membrane protein How can you get membrane protein from E. coli and how to separate the inner and outer membrane protein?
What is inner membrane protein and outer membrane protein How can you get membrane protein from Ecoli and how to separate the inner and outer membrane protein email helen110559@sina.com
What is the full name of E. Coli?
Escherichia coli
What is the scientific name for escherichia coli?
domain:bacteria kingdom:eubacteriaphylum:proteobacteriaclass:gamma proteobacteriaorder:enterobacterialesfamily:enterobacteriaceaegenus:escherichiaspecies:E. coli
What is E. coli bl21?
an E. coli B strain, usually used for protein-expression.
How can protein be expressed in bacteria?
The first choice for the expression of recombinant proteins is typically E. coli. Production of proteins in this bacterial strain is well-established, fast and simple and usually provides high yields. Recent progress in the fundamental understanding of transcription, translation, and protein folding in E. coli, improved genetic tools had made this bacterium more valuable than ever for the expression of complex eukaryotic proteins. This is what I learned on Creative Biolabs.
What is H4S8O3?
It is a protein found in E-coli bacteria.
How can you get membrane protein from E. coli?
What is inner membrane protein and outer membrane protein How can you get membrane protein from Ecoli and how to separate the inner and outer membrane protein.
What is MDG medium?
It's a non-inducing medium for growing bacteria, particularly Escherichia coli strains used for protein production. It was formulated by Studier and published in 2005 (Protein Expression and Purification 41: 207-234 (2005) ). The composition of MDG is freely available on the internet.
What bacteria contains seravars based on the h protein?
E. Coli
How do you show protein expression in bacteria?
The first choice for the expression of recombinant proteins is typically E. coli. Production of proteins in this bacterial strain is well-established, fast and simple and usually provides high yields. Recent progress in the fundamental understanding of transcription, translation, and protein folding in E. coli, improved genetic tools had made this bacterium more valuable than ever for the expression of complex eukaryotic proteins.
What is the lac operon in E. coli a classic example of?
gene expression occurring at transcription
What has the author Matthew L Nilles written?
Matthew L. Nilles has written: 'Regulation of the acrAB operon of Escherichia coli' -- subject(s): Genetics, Multidrug resistance, Escherichia coli, Gene expression, Operons
How does E. coli benefit by making enzymes to utilize lactose only when lactose is in the cellular environment?
By controlling gene expression, E. coli bacteria conserve resources and produce only those proteins that are needed
What has the author Soonhee Park written?
Soonhee Park has written: 'Genetic and biochemical studies on the export of ribose-binding protein in Escherichia coli' -- subject(s): Genetics, Proteins, Escherichia coli, Synthesis
What is inner membrane protein and outer membrane protein How can you get membrane protein from E. coli and how to separate the inner and outer membrane protein?
What is inner membrane protein and outer membrane protein How can you get membrane protein from Ecoli and how to separate the inner and outer membrane protein email helen110559@sina.com
