Wiki User
∙ 15y agoAlways the bottom layer will be of chloroform and upper layer of water due to difference in the density.
Wiki User
∙ 15y agoYou can quickly confirm which layer is water and which is chloroform by adding a few drops of water to a test tube containing the layers. Water will mix with the layer that is water, causing it to become more transparent. Chloroform will not mix with water and remain as a separate layer.