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What are some factors that may influence enzyme activity?

Updated: 8/16/2019
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13y ago

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Factors affecting enzyme activity1: Enzyme concentration

If the quantity of enzyme is doubled, the enzymatic activity will also be doubled because more enzymes are now available to work. After a certain level of enzyme concentration, there will be no more increase in the enzymatic activity because all the substrate molecules are combined with an enzyme and the rate of reaction will stabilize.

2: Substrate concentration

By increasing substrate concentration, enzymatic activity increases. Increasing the substrate further without increasing the enzyme concentration will not affect the enzymatic activity because all the enzymes are occupied by a substrate molecule.

3: pH value

Some enzymes require acidic surroundings, most require a more neutral condition for their activity. Change in the pH can change the enzyme's structure and enzyme become useless.

4: Temperature

An increase in temperature of 10 degree celsius doubles the enzymatic activity. Each enzyme has its own optimum temperature at which its enzymatic activity is maximum. Very high temperatures break the bonds that maintain shape of enzyme. If the enzyme denatures, the substrate can not fit in to the active sites and enzyme become useless.

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pH, temperature

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