If the quantity of enzyme is doubled, the enzymatic activity will also be doubled because more enzymes are now available to work. After a certain level of enzyme concentration, there will be no more increase in the enzymatic activity because all the substrate molecules are combined with an enzyme and the rate of reaction will stabilize.
2: Substrate concentration
By increasing substrate concentration, enzymatic activity increases. Increasing the substrate further without increasing the enzyme concentration will not affect the enzymatic activity because all the enzymes are occupied by a substrate molecule.
3: pH value
Some enzymes require acidic surroundings, most require a more neutral condition for their activity. Change in the pH can change the enzyme's structure and enzyme become useless.
4: Temperature
An increase in temperature of 10 degree celsius doubles the enzymatic activity. Each enzyme has its own optimum temperature at which its enzymatic activity is maximum. Very high temperatures break the bonds that maintain shape of enzyme. If the enzyme denatures, the substrate can not fit in to the active sites and enzyme become useless.
pH, temperature
Enzymes are catalysts that speed up chemical reactions. Some environmental factors that can affect enzyme activity are temperature and pH levels.
Blocking of enzyme activity depends on various factors such as concentration of substrates, pH, temperature, some durgs. As for example rifampicin durg blocks RNA polymerase II activity.
A low temperature can slow down enzyme activity and high temperatures can denature an enzyme making it unusable. pH levels also affect enzyme activity. Every cell has an ideal temperature and pH
This depends on the enzyme. Temperature, pH, and the presence of co-factors, or allosteric inhibitors.
Non-protein compounds called co-factors are required for some proteins in order to facilitate enzymatic activity. Common examples of co-factors are metal ions (Ca2+, Mg2+ etc.), organic co-factors such as heme (in hemoglobin), among others.
have a shape that fits into the enzyme
One is cofactor A.
Substance concentration, enzyme concentration, temperature and PH level
Temperature, rainfall, vegetation, animal activity, type of rock being weathered.
Hydrogen peroxide (H2O2) is a poisonous byproduct of metabolism that can damage cells if it is not removed. Catalase is an enzyme that speeds up the breakdown of hydrogen peroxide into water (H2O) and oxygen gas (O2).
Locamotion, cell movement, biosynthesis
Some of the factors that influence the HNS operations are logistics, the personnel and dependability. Other factors are competition and the availability of the local resources.