Agar deeps are used to see whether an organism requires oxygen to grow. If there is spreading growth only at the bottom of the tube, the organism is an obligate anaerobe (meaning it cannot tolerate oxygen). If there is growth only at the surface of the agar, the organism is an obligate aerobe (it cannot grow without oxygen). And if there is growth all along the point of innoculation, the organism is a facultative anaerobe and can survive either way.
An agar deep is used to find out the gas requirements of an microbe. Does it thrive in oxygen? Or in oxygen lacking enviroments? After inoculating the microbe in an agar deep, look at the results. If it only grows on the surface, it means the microbe requires oxygen. But if it can also grow within the deep, below the surface, it shows the microbe is capable or surviving in low oxygen concentration. Lastly, if the microbe is found growing far away from the surface, it means that this microbe grows in anaerobic conditions Hope this helps. From, A student studying microbiology
The primary purpose of an agar deep is to find out what types of gas are required for a microbe. When a microbe grows on the surface this signifies that the microbe requires oxygen for survival.
To identify if the bacteria will grow aerobically or not.
inoculation needle
This is important to prevent the inoculating needle from becoming stuck in the agar, taking out pieces of agar while trying to remove the instrument. This agar will get into the inoculum when sterilizing the needle on the flame, causing contamination to your sample.
Yes! Paenibacillus spp. can degrade agar agar
I teaspoon Agar Agar = 2 grams
red algae Agar agar is a malay term meaning red algae. This algae which agar is extracted lives in the ocean around Malaysia
agar is botained from a red alga called gelidium
Agar deep refers to a measure used with microbes. It is a measurement of how much gas a microbe requires for survival.
inoculation needle
agar formation.
Over time, free oxygen will diffuse into the agar.
Agar is a solid form of nutrient for different strains of bacteria. Anyone who needs to grow bacteria for use in their labs or experiments would use agar.
a slant broth is a type of inoculating agar to gather bacterial data from.
Because the peptone iron agar is used to detect ANAEROBIC bacteria. If you stab it deep into the agar you allow the bacteria to grow in the absence of oxygen. If you only inoculated the surface the bacteria wouldn't grow.
Simmons citrate agar is a differential agar used to determine if a sample bacteria can utilize citrate as its only carbon source. The agar is initially a green color due to the bromo thymol blue pH indicator in it. If a bacteria uses the citrate, the by-products are ammonia and ammonium hydroxide both of which will alkalize the agar and increase the pH to the point of changing the indicator's color to blue, so the whole agar turns from green to blue.
Agar, or agar-agar, is not a grain, but rather an extract of seaweed. Agar translates to German as Agar-Agar Try whole- or health-food stores
This is important to prevent the inoculating needle from becoming stuck in the agar, taking out pieces of agar while trying to remove the instrument. This agar will get into the inoculum when sterilizing the needle on the flame, causing contamination to your sample.
Yes! Paenibacillus spp. can degrade agar agar