What does a buffer do in chemistry?
A buffer maintain constant the pH.
As a noun, "buffer" in chemistry means a mixture of at least two materials that resists changes in pH value when small amounts of acid or base are added to the mixture. As a verb, "buffer" in chemistry means to add at least one substance to a substance or mixture that is not originally a buffer that will cause the mixture formed by the addition to become a buffer in the noun sense.
I think you mean electrphoresis buffer ( anyway the principle is the same for all sloutions,, simply it is chemistry ) if so , you'll need to take about 25 ml from your 40X buffer and complete them till 1000 ml with distilled water. The calculation can be done as the following N * V = N' * V' so 40X * Z = 1X * 1000 Z is the amount of 40X buffer needed…
The buffer capacity increases as the concentration of the buffer solution increases and is a maximum when the pH is equal to the same value as the pKa of the weak acid in the buffer. A buffer solution is a good buffer in the pH range that is + or - 1 pH unit of the pKa. Beyond that, buffering capacity is minimal.
it is defined the capability of a buffer to resist the change of pH.it can be measured quantity that how much extra acid or base , the solution can absorb before the buffer is essentially destroyed. buffer capacity of a buffer solution is determined by the sizes of actual molarities . so , a chemist must decide before making the buffer solution.
Buffer is a combination of acid/base and its salt, bicarbonate is an acid buffer which means H2CO3 and Na or K bicarbonate together act as a buffer. When you add acid proton(H+) is taken by the bicarbonate ion and if you add base OH- ion is taken by the Na or k in the buffer, in that way buffer acts.