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A recombination frequency is a measure of how likely it is that two genes are linked. It will also tell how likely it is that a crossing over event will occur between those two genes. Thus, for an RF=50% it is random as to whether or not the genes will be inherited together or whether crossing over will separate them (i.e. they independently assort). Traditionally, following Mendel's Second Law (that alleles of genes independently assort), an RF=50% means that the two genes are on different chromosomes; a slightly more complicated corollary is that the two genes need not be on different chromosomes but may be on the same chromosome just very far apart from each other. Thus, the smaller the RF (maximum RF is 50%), the more likely it is that two genes are linked.

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