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how to make sodium citrate in 10% ethanol for DNA extraction
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What is the function sodium perchlorate in extraction DNA from blood?
Sodium perchlorate is used to deproteinize your DNA preps. At high concentrations, Na perchlorate will remove SDS and associated proteins and prevent proteins from precipitating with your nucleic acid in your ethanol ppt step.
What is the function of liquid detergent in genomic DNA extraction?
Liquid detergent used in the genomic DNA extraction, emulsify plasma membrane and nuclear membrane promoting lysis. SDS (Sodium Dodecyl Sulphate) is an anionic detergent used in DNA extraction. It removes the positive ions from the proteins, due to this protein loses its conformation and gets destroyed thus the cell membrane gets damaged and cell gets broken.
What tube is use to collect blood for DNA?
Light yellow top tube contains the additive sodium polyanethol sulfonate (SPS) or acid citrate dextrose additives. This tube is used for blood bank studies, human leukocyte antigen (HLA) phenotyping, DNA, and paternity testing.
What does salt do in DNA extraction?
The salt neutralizes the DNA's negative charge with its positive charge while the DNA precipitates.
Why pH of Simmon's citrate agar increase if citrate is utilized?
This is due to the production of oxalacetic acid and acetate, when the enzyme citrase acts on the citrate. the oxalacetic acid and acetate is enzymatically converted to pyruvic acid and carbon dioxide. the carbon dioxide combines with sodium and water forming sodium carbonate which in turns makes the medium alkaline.
Can i use saline citrate buffer for DNA extraction from tissue of bivalve?
yes??
Sodium acetate in plant DNA extraction?
yes
Why you add sodium acetate in dna extraction?
you add sodium acetate to neutralize the charge on the sugar-phosphate backbone of DNA
What is the function of sodium citrate in DNA isolation?
Sodium ions neutralize the negative charge of the DNA backbone (phosphates) making the DNA less hydrophilic (less likely to be solubilized) in the wash solution
What type of bond is sodium citrate?
sodium citrate bond
Can you use trisodium citrate insteade of sodium citrate 2 hydrate?
yes you can use either trisodium citrate or sodium citrate
What is the purpose of NACL is DNA extraction?
Sodium chloride help the separation of DNA from other proteins.
Is sodium citrate an electrolyte?
No it's not caustic. In fact the pH is about 7
What is the purpose of using sodium chloride in DNA extraction experiment?
if is the best known example mixture
What is the role of sodium acetate in DNA extraction?
sodiom acetat reaction with membrane protein and cause that persipitate and help to dna isolation
Is the source of sodium citrate natural?
Yes, the source of sodium citrate is natural. Sodium citrate occurs naturally in citrus fruits such as pineapples, lemons and oranges.
What is the dfference between sodium citrate and sodium citrate dihydrate?
two water molecules
