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What is LST Broth?

Updated: 11/2/2022
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Lauryl Sulfate Tryptose (LST) Broth, are used for the detection of coliform organisms in materials or foods .

This medium is used for the detection of coliforms in foods and dairy products

Lauryl Tryptose Broth

Approximate Formula* Per Liter

Tryptose .................................................................... 20.0 g

Lactose ....................................................................... 5.0 g

Dipotassium Phosphate............................................... 2.75 g

Monopotassium Phosphate......................................... 2.75 g

Sodium Chloride ......................................................... 5.0 g

Sodium Lauryl Sulfate.................................................. 0.1 g

Peptone provides essential growth substances, such as nitrogen and carbon compounds, sulfur and trace ingredients. The

potassium phosphates provide buffering capacity. Sodium

chloride maintains osmotic equilibrium.

Lactose provides a source of fermentable carbohydrate

for coliform organisms. The fermentation of lactose with gas

formation is a presumptive test for coliforms. Sodium lauryl

sulfate inhibits organisms other than coliforms

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