In paper chromatography n-butanol:acetic acid:water mixture in the ratio 4:1:1 is used as a solvent whereas in case of thin layer chromatography(TLC) petroleum ether and acetone in the ratio 9:1 is used as a solvent.
Also, in some cases, DCM(dichloromethane is used as a solvent.
As such there is no hard and fast rule which solution to use. It depends on what component of the material are we interested in as carries the components of the mixture with it and different components travel at different rates.
i dont know what your talking about dude!
whatmann filter paper
Black ink
The end of the paper nearest the spot is allowed to be in contact with a suitable solvent, WITHOUT immersing the spot itself. If the spots were immersed they would dissolve in the solvent and be pulled down into the solvent itself.
the more soluble the plant pigment is with the chromatography solvent, the farther it will travel up the chromatography paper
Because methanol is a very polar solvent
The Rf value cannot be determined since the distance of the solvent may overlay in the paper.
Chromatography is a method of analyzing the contents of a mixed substance. It is performed by dissolving the substance in a suitable solvent. A drop or spot of the resulting liquid is deposited near the edge of a piece of absorbent paper, such as blotting paper or special chromatography paper. The strip of paper with the spot at the bottom is then suspended over a solvent with the bottom edge of the paper in the solvent. As the solvent soaks upwards, the solvent carries some particles upwards and away from the spot. Different compounds within the mixture will travel at different rates and eventually the strip of paper will show different bands of colors, separating out and indicating some of the various components that were in the original substance. 'Trailing a spot' is a short term for this laboratory process.
The end of the paper nearest the spot is allowed to be in contact with a suitable solvent, WITHOUT immersing the spot itself. If the spots were immersed they would dissolve in the solvent and be pulled down into the solvent itself.
In chromatography you need one stationery phase (paper) and one mobile phase (here solvent, moving by capillary forces)
the more soluble the plant pigment is with the chromatography solvent, the farther it will travel up the chromatography paper
Because methanol is a very polar solvent
The Rf value cannot be determined since the distance of the solvent may overlay in the paper.
This method is called paper chromatography.
The water dissolves the paper molecules.
Chromatography is a method of analyzing the contents of a mixed substance. It is performed by dissolving the substance in a suitable solvent. A drop or spot of the resulting liquid is deposited near the edge of a piece of absorbent paper, such as blotting paper or special chromatography paper. The strip of paper with the spot at the bottom is then suspended over a solvent with the bottom edge of the paper in the solvent. As the solvent soaks upwards, the solvent carries some particles upwards and away from the spot. Different compounds within the mixture will travel at different rates and eventually the strip of paper will show different bands of colors, separating out and indicating some of the various components that were in the original substance. 'Trailing a spot' is a short term for this laboratory process.
A hypothesis for paper chromatography depends on what you are making the hypothesis on. A hypothesis for the speed of chromatography could be that you think the speed of the process can be changed depending on the type of paper, or whatever the stationary phase is, and the type of solvent being used.
Ascending paper chromatography is where a chromatogram rises, or ascends. The paper moves up because the solvent moves it in an upward direction.
Ascending paper chromatography is where a chromatogram rises, or ascends. The paper moves up because the solvent moves it in an upward direction.
They do not move up because the aren't soluble the solvent