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Protein assay is the determination of concentration or total level of protein in a solution.There are various protein assays employed like bradford assay and lowry assay
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What is a bandshift assay?
A bandshift assay is a type of assay using gel electrophoresis, in which the mobility of a DNA or RNA probe alone is compared to its mobility in combination with a particular protein.
Is the Bradford dye method of protein assay considered destructive or non destructive?
non-destructive
Merits and demerits of Bradford assay?
Bradford protein assay is a very quick way of testing for proteins in solutions. However, presence of detergents in the solution being investigated renders this test inapplicable.
What is azocasein?
Azocasein is a chemically modified protein that was designed as a substrate for quantative assay of proteolytic enzyme activity. Hope that helped!!
What is a marker protien?
A protein marker is just that - a marker for specific proteins. This usually deals with running an experiment (assay) to determine the presence, absence, and with some markers, abundance of a specific protein
What is a p24 antigen capture assay?
Found in the viral core of HIV, p24 is a protein that can be measured by the ELISA technique. Doctors can use p24 assays to measure the antiviral activity of the patient's medications. In addition, the p24 assay is sometimes useful.
What is an assay?
An assaying is a trial by assay, thorough examination and determination.
How does EMSA electromobility shift assay measure whether the protein bends DNA?
EMSA does not measure if protein bends to DNA. It does measure what proteins bind to a specific region of DNA (usually a promoter region). You can use a supershift to determine exactly what protein is binding to the specific DNA region.
To test wether a sample contains proteins what reagent should be used?
The Bradford reagent (Coomassie) is commonly used to detect if a sample contains protein. Coomassie will react with aromatic amino acids (phenylalanine, tyrosine and tryptophan) to turn from a dull red color to a bright blue color. This assay is dependant on the amount of aromatic amino acids present, but works well as a "quick and dirty" indicator of the presence of a protein. The bicinchonic acid assay (BCA assay), while more expensive than the Bradford assay, more accurately detects the presence of the peptide bond present in proteins, so it can be used to not only detect proteins which lack aromatic amino acid residues, but also can be used to more accurately determine the concentration of protein in a sample as not all proteins have the same amount of aromatic amino acids.
How do you verify gold bar assay certificate?
You can't verify assay certificates.
What is the advantages of using biuret reaction in place of measuring the absorption of the protein solution at 280 nm?
The absorption of proteins at 280nm is according to electrons from the rings on the amino acid such as His, Trp, etc. And if there is no such kind of amino acids in the protein, we might not be able to get what the amount of the protein really is. At the other hand, what makes biuret reaction work is by the copper ion reacting with the dipeptide bonds, since every amino acid has the peptide bond, it's more accurate and reasonable to use biuret reactions to determine what the amount of the protein is.
When was US Assay Commission created?
United States Assay Commission was created in 1792.
