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What is the difference between Restriction endonucleases and exonucleases?
Wiki User
∙ 14y ago
Exonuclease enzymes cleave nucleotides from the ends of DNA molecules.
Endonuclease enzymes cleave a phosphodiester bond somewhere within the DNA molecule (not at the ends).
Wiki User
∙ 14y ago
Wiki User
∙ 11y agoNo restriction endonucleases is one of the examples for restriction enzymes.
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Describe the difference between a single digest and a double digest?
Restriction enzymes (endonucleases) are used for a variety of reasons in molecular genetics, including obtaining a "map" and cloning DNA. Single digests consitute DNA being treated with one restriction endonuclease, whereas double digests contain 2 enzymes. At times, it is difficult (or not possible) to perform double digests ... especially when the 2 enzymes have very different requirements for their activities (e.g. salt concentration, temperature optimums, ...). If a DNA restriction map is known for a particular enzyme, and if the DNA is treated with this enzyme, then one can ascertain whether the digest was complete or not. However, if a restrictioin map is just being compiled, and if the DNA is treated with 2 enzymes in a double digest, at times difficulties may arise in determining the map if either (or both) enzymes did not completely digest the DNA.
What is the difference between blow off valves and waste gates?
the bov allows excess boost/pressure to out of the intake when the throttle body is closed. A wastegate is on the exhaust side and allows exhaust gas to bypass the turbocharger at high rpm, this reduces the restriction of airflow by the turbo.
What is the difference between technology and process?
what is the difference between license and patent
What is the difference between an assembler and a translator?
what is the difference between an assembler and the translator
What is the Difference between HVAC and AHU?
What is difference between hvac and ahu
What are isochizomers?
Isochizomers are such restriction endonucleases which have the same recognition sequence but may have different recognition site. examples are XmaI and SmaI which have same recognition sequence 5'CCCGGG3' but SmaI cuts between C and G and XmaI cuts between first C and second C.
What are restriction enzymes?
Restriction enzymes (also known as restriction endonucleases) are proteins which cut DNA up at specific sequences in the genome. For example, the commonly used restriction endonuclease EcoRI recognizes every point in DNA with the sequence GAATTC, and cuts at the point between the Guanine and Adenine. Interestingly, the recognition sequences for most restriction endonucleases are genetic palindromes, e.g., the sequence reads exactly the same backwards on the complementary strand. In the case of EcoRI, the two complementary DNA strands for the recognition sequence are: 5'--GAATTC ---3'3'--CTTAAG--5'
Why is it important to use the same restriction enzyme for both cells in recombinant DNA?
Restriction enzymes are endonucleases that digest the DNA at a sequence specific site. Hind III for example cut between two As in the sequence AAGCTT in the both strand forming a sticky end. If you use this enzyme to cut in your vector DNA, you have to use the same enzyme in the insert DNA so as they can ligate by DNA ligation. This is the important use of same restriction enzyme in cloning.
What cuts DNA at a specific sequence of nucleotides?
Restriction enzyme cut the DNA at the specific site. Xho I is an example for restriction endonuclease which cut between C and T in the sequence of -CTCGAG- at the both strands. This is highly specific and hence they are used in DNA or gene cloning.
How do restriction sites and a restriction map relate?
Restriction maps show the lengths of DNA fragments between restriction sites in a strand of DNA.
What is the difference between resistor and insulator?
A resistor lets electricity flow, but in a calculated restriction of amount. An insulator prevents electricity from flowing across it - period.
What is the restriction site of the restriction enzyme Hae III?
The restriction site of Hae III is GGCC. It cuts between the G and the C. This produces blunt ends.
How are restriction maps used?
They are used to show the lengths of DNA fragments between restriction sites in a strand of DNA.
Where is the governor on a Honda metropolitan?
check the exhaust and google 'restriction' The restriction plate is in between the carburetor and the air intake
Describe the difference between a single digest and a double digest?
Restriction enzymes (endonucleases) are used for a variety of reasons in molecular genetics, including obtaining a "map" and cloning DNA. Single digests consitute DNA being treated with one restriction endonuclease, whereas double digests contain 2 enzymes. At times, it is difficult (or not possible) to perform double digests ... especially when the 2 enzymes have very different requirements for their activities (e.g. salt concentration, temperature optimums, ...). If a DNA restriction map is known for a particular enzyme, and if the DNA is treated with this enzyme, then one can ascertain whether the digest was complete or not. However, if a restrictioin map is just being compiled, and if the DNA is treated with 2 enzymes in a double digest, at times difficulties may arise in determining the map if either (or both) enzymes did not completely digest the DNA.
What are the noise restriction levels?
The threshold of pain with a sound pressure level between 120 and 140 dBSPL is really the restriction limit.
Difference between aggregation and composition?
Aggregation is a collection of objects where they are loosely associated with each other. Composition is when there is a tighter restriction between two, or more, objects where the composed object cannot exist without the other object, or objects.
