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What problem will ensue if the level of the developing liquid is higher than the applied spot in a TLC analysis?
Wiki User
∙ 11y ago
The effect on chromatographic work if the solvent level in the developing chamber is higher than the spotted sample is a thin layer chromatography. The solvent becomes polar enough and spot will move some distance.
Wiki User
∙ 9y ago
Wiki User
∙ 11y agoIf the spots are submerged in the solvent, they are washed off the plate and lost.
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What problem will ensue if the level of developing liquid is higher than the applied spot in a TLC analysis?
If the spots are submerged in the solvent, they are washed off the plate and lost.
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