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The essence of making a thin smear is to spread a sample of cells, bacteria, or other substances onto a slide in a very thin layer. This allows for microscopic examination of the sample, so that individual cells and components can be observed. Making a thin smear involves taking a small sample of the material, suspending it in a liquid, and using a microscope slide to spread the sample evenly across the slide. The sample must be spread thinly enough so that individual cells are visible. When the slide is stained, the thin smear allows individual cells to be identified and counted. Making a thin smear is a fundamental part of many laboratory procedures, such as microscopic examination of blood or body fluids, or bacterial culturing.
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Shape of a human blood smear?
A human blood smear typically has a thin, elongated shape on a glass slide. The thin layer of blood is spread evenly across the slide to allow for visual examination under a microscope. This shape allows for a clear view of the different types of blood cells present in the sample.
Give the reason for passing bacterial smear through the flame before staining?
Passing the bacterial smear through the flame before staining is done to heat-fix the bacteria onto the slide, making them adhere firmly and preventing them from washing off during the staining process. Heat fixing also kills the bacteria, which helps in the preservation of their cellular structures for visualization under the microscope.
How can incorrect results with Gram-variable can be avoided?
Run a positive and negative control organism on the same slide with the unknown Make a thin smear Pay close attention when decolorizing and rinse with water as soon as bleeding stops
What are the precautions during preparation of bacterial smear and heat -fixing process?
During the preparation of a bacterial smear, it is crucial to use sterile techniques to avoid contamination. Use a sterile inoculating loop to transfer a small amount of culture onto a clean slide, and ensure the smear is thin and evenly spread. When heat-fixing, gently pass the slide through a flame to adhere the bacteria to the slide without burning them, as excessive heat can distort cellular structures. Always wear gloves and goggles to protect yourself from potential biohazards.
What is the effect of heating the smear flooded with a carbol fuchsin stain?
Heating the smear flooded with carbol fuchsin stain helps in the penetration of the stain into the bacterial cell wall by softening the cell wall and making it more permeable. This process is important for the retention of the stain during the subsequent decolorization step in the staining process.
What is the important of thin blood smear?
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What is the effect of a very thin preparation in fecal smear?
no sign from the teacher.
Is there any difference if the prepared smear is too thick or too thin?
Thin smears of blood are needed to investigate hematological problems or disorders of the blood. It is also used to identify the parasite within the blood. Thick films enables the microscopist to screen the blood of a larger volume. They are more sensitive than the thin film.
Shape of a human blood smear?
A human blood smear typically has a thin, elongated shape on a glass slide. The thin layer of blood is spread evenly across the slide to allow for visual examination under a microscope. This shape allows for a clear view of the different types of blood cells present in the sample.
What modern facilities are making the ozone thin?
CFC's are making ozone thin. They are man made chemicals.
What is the essence or core?
essence in runescape is used for making runes - runes are used by magic fighters in battle. U can mine essence in special places like ores
What does the imp catcher quest in runescape give you?
It gives you access to the essence mines, where you can mine Rune Essence ( non members way of making money ) or Pure Essence ( Members Way of making money ), alternatively the essence you mine can be crafted into runes using the runecrafting skill, and the appropriate altar and talisman.
What would be the result of overheating the bacterial smear?
Overheating the bacterial smear can result in distortion or destruction of the bacterial cells, making it difficult to observe them under the microscope. This can lead to inaccurate or inconclusive results when trying to identify the bacteria present on the smear.
What is the best powerbot script for money making?
pure essence
What does the term smear means in microbiology?
In microbiology, smear refers to the process of spreading a sample (such as bacteria or cells) onto a microscope slide for staining and observation. Smears are used to create a thin, even layer of the specimen for better visualization under the microscope.
How do you get a sample of thin pap smear?
The Thin Prep collection process allows you to use a broom or a traditional Ayres spatula and brush. You collect the sample as with other pap smear collection devices. The important thing is vigorous agitation of the collection device(s) in the fluid, as well as pressure of the cytobrush against the walls of the vial to remove as many cells as possible from the brush.
What will happen when a thick smear is stained and viewed under a microscope?
When a thick smear is stained and viewed under a microscope, individual cells will be harder to distinguish due to overlapping. This can make it challenging to see details within individual cells, such as the internal structures or boundaries. It is typically recommended to use a thin smear for better visualization and interpretation of cellular details.
