RNA is insoluble in isopropanol so it will aggregate together, giving a pellet upon centrifugation. This step also removes alcohol-soluble salt.
Its main role is to pricipitate the DNA, by engaging with the water molecule as not giving chance for the DNA to get dissolve in the water.
Precipitates DNA and separation is carried out...
DNA is not soluble in isopropyl alcohol. It will precipitate out when you add this solvent. Once out of solution you can centrifuge it down and collect the pellet of DNA.
Isopropanol is more preferred than ethanol in DNA extraction, as isopropanol facilitates precipitation more better, as it possess very less i.e., 0.6 to 0.7 volumes of alcohol.
RNAse destroys the RNA and hence RNAse contamination is a problem in RNA extraction as it breaks down RNA. RNAse enzyme is removed by using RNAse inhibitor or precautions like wearing of gloves, autoclaving tips , using RNAse free water/DEPC treated water is done while performing RTPCR
Isopropanol precipitates the RNA. Up to that point it's generally in solution. Centrifuging the tube after this step should leave a very faint but generally visible white smudge/pellet of RNA. The ethanol steps that follow the isopropanol precipitation are simple washes.
To concentrate or purify the DNA, which is insoluble in isopropanol. Once the solution containing your DNA is placed in isopropanol and centrifuged, the DNA will precipitate to a little pellet at the bottom of your tube. Everything else in your tube is soluble in isopropanol and will remain in liquid form. Pipet the liquid out and now you have just DNA.
It inactivate the RNase and prevent RNA to denature.
chloroform is used to denature protein and settle it in the bottom during rna extraction ,also it helps to form organic and inorganic layers in which rna is dissolved in inorganic layer.
Isopropanol is more preferred than ethanol in DNA extraction, as isopropanol facilitates precipitation more better, as it possess very less i.e., 0.6 to 0.7 volumes of alcohol.
RNAse destroys the RNA and hence RNAse contamination is a problem in RNA extraction as it breaks down RNA. RNAse enzyme is removed by using RNAse inhibitor or precautions like wearing of gloves, autoclaving tips , using RNAse free water/DEPC treated water is done while performing RTPCR
Isopropanol precipitates the RNA. Up to that point it's generally in solution. Centrifuging the tube after this step should leave a very faint but generally visible white smudge/pellet of RNA. The ethanol steps that follow the isopropanol precipitation are simple washes.
TE stands for Tris and EDTA. The Tris buffers the water to prevent acid hydrolysis of the DNA/RNA. The EDTA chelates divalent cations that can assist in the degradation of RNA.
To concentrate or purify the DNA, which is insoluble in isopropanol. Once the solution containing your DNA is placed in isopropanol and centrifuged, the DNA will precipitate to a little pellet at the bottom of your tube. Everything else in your tube is soluble in isopropanol and will remain in liquid form. Pipet the liquid out and now you have just DNA.
Proteins, RNA, lipids
to precipitate protein.
In a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.
I do my RNA extractions at pH 5.0. I think it depends on the method that you use. You will have to say what method that you use to do your RNA extractions.
The density of 70% ethanol allows RNA settlement or say sedimentation in the vial.
You want bands. The bands are ribosmal RNA of various sizes. Bands are good this shows that you did a good job of extracting RNA.